Abstract
The pattern of protein synthesis was compared in several organs of maize (Zea mays L.) under aerobic and anaerobic conditions. Protein synthesis was measured by [35S]methionine incorporation and analysis by two-dimensional native-SDS (sodium lauryl sulfate) polyacrylamide gel electrophoresis and fluorography. The aerobic protein-synthesis profiles were very different for root, endosperm, scutellum and anther wall. However, except for some characteristic qualitative and quantitative differences, the patterns of protein synthesis during anaerobiosis were remarkably similar for these diverse organs and also for mesocotyl and coleoptile. The proteins synthesized were the anaerobic polypeptides (ANPs) which have been previously described in anaerobic roots of seedlings. Leaves exhibited no detectable protein synthesis under anaerobic conditions, and died after a short anaerobic treatment. Evidence is presented that the ANPs are not a generalized response to stress. This indicates that the ANPs are synthesized as a specific response to anaerobic conditions such as flooding.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 89-94 |
| Number of pages | 6 |
| Journal | Planta |
| Volume | 150 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 1 1980 |
| Externally published | Yes |
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Keywords
- Anaerobiosis
- Protein synthesis, organspecific
- Stress
- Zea
ASJC Scopus subject areas
- Genetics
- Plant Science
Cite this
Patterns of polypeptide synthesis in various maize organs under anaerobiosis. / Okimoto, Ronald; Sachs, Martin M.; Porter, Elizabeth K.; Freeling, Michael.
In: Planta, Vol. 150, No. 1, 01.11.1980, p. 89-94.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Patterns of polypeptide synthesis in various maize organs under anaerobiosis
AU - Okimoto, Ronald
AU - Sachs, Martin M.
AU - Porter, Elizabeth K.
AU - Freeling, Michael
PY - 1980/11/1
Y1 - 1980/11/1
N2 - The pattern of protein synthesis was compared in several organs of maize (Zea mays L.) under aerobic and anaerobic conditions. Protein synthesis was measured by [35S]methionine incorporation and analysis by two-dimensional native-SDS (sodium lauryl sulfate) polyacrylamide gel electrophoresis and fluorography. The aerobic protein-synthesis profiles were very different for root, endosperm, scutellum and anther wall. However, except for some characteristic qualitative and quantitative differences, the patterns of protein synthesis during anaerobiosis were remarkably similar for these diverse organs and also for mesocotyl and coleoptile. The proteins synthesized were the anaerobic polypeptides (ANPs) which have been previously described in anaerobic roots of seedlings. Leaves exhibited no detectable protein synthesis under anaerobic conditions, and died after a short anaerobic treatment. Evidence is presented that the ANPs are not a generalized response to stress. This indicates that the ANPs are synthesized as a specific response to anaerobic conditions such as flooding.
AB - The pattern of protein synthesis was compared in several organs of maize (Zea mays L.) under aerobic and anaerobic conditions. Protein synthesis was measured by [35S]methionine incorporation and analysis by two-dimensional native-SDS (sodium lauryl sulfate) polyacrylamide gel electrophoresis and fluorography. The aerobic protein-synthesis profiles were very different for root, endosperm, scutellum and anther wall. However, except for some characteristic qualitative and quantitative differences, the patterns of protein synthesis during anaerobiosis were remarkably similar for these diverse organs and also for mesocotyl and coleoptile. The proteins synthesized were the anaerobic polypeptides (ANPs) which have been previously described in anaerobic roots of seedlings. Leaves exhibited no detectable protein synthesis under anaerobic conditions, and died after a short anaerobic treatment. Evidence is presented that the ANPs are not a generalized response to stress. This indicates that the ANPs are synthesized as a specific response to anaerobic conditions such as flooding.
KW - Anaerobiosis
KW - Protein synthesis, organspecific
KW - Stress
KW - Zea
UR - http://www.scopus.com/inward/record.url?scp=10944233132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10944233132&partnerID=8YFLogxK
U2 - 10.1007/BF00385619
DO - 10.1007/BF00385619
M3 - Article
C2 - 24306530
AN - SCOPUS:10944233132
VL - 150
SP - 89
EP - 94
JO - Planta
JF - Planta
SN - 0032-0935
IS - 1
ER -