Patterning cells and their environments using multiple laminar fluid flows in capillary networks

Shuichi Takayama, J. Cooper Mcdonald, Emanuele Ostuni, Michael N. Liang, Paul J.A. Kenis, Rustem F. Ismagilov, George M. Whitesides

Research output: Contribution to journalArticlepeer-review


This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.

Original languageEnglish (US)
Pages (from-to)5545-5548
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
StatePublished - May 11 1999
Externally publishedYes

ASJC Scopus subject areas

  • General

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