Pathway engineering as an enabling synthetic biology tool

Dawn T. Eriksen; Sijin Li; Huimin Zhao

doi:10.1016/B978-0-12-394430-6.00003-0

Pathway engineering as an enabling synthetic biology tool

Dawn T. Eriksen, Sijin Li, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Microbial synthesis of value-added compounds often depends upon the creation or introduction of heterologous metabolic pathways into production hosts. However, there are many challenges in the engineering of a pathway and even more issues in optimizing it for the highest product yield. In this chapter, we will review a wide variety of computational and experimental tools that have been developed to design, construct, and optimize metabolic pathways. In addition, we will illustrate the applications of these tools in synthetic biology by highlighting the engineering of three metabolic pathways involved in the synthesis of glucaric acid, 1,4-butanediol, and 1,3-propanediol. © 2013

Original language	English (US)
Title of host publication	Synthetic Biology
Publisher	Elsevier Inc.
Pages	43-61
Number of pages	19
ISBN (Print)	9780123944306
DOIs	https://doi.org/10.1016/B978-0-12-394430-6.00003-0
State	Published - 2013

Keywords

DNA assembly
Metabolic flux balancing
Pathway engineering
Pathway optimization

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Online availability

10.1016/B978-0-12-394430-6.00003-0

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abstract = "Microbial synthesis of value-added compounds often depends upon the creation or introduction of heterologous metabolic pathways into production hosts. However, there are many challenges in the engineering of a pathway and even more issues in optimizing it for the highest product yield. In this chapter, we will review a wide variety of computational and experimental tools that have been developed to design, construct, and optimize metabolic pathways. In addition, we will illustrate the applications of these tools in synthetic biology by highlighting the engineering of three metabolic pathways involved in the synthesis of glucaric acid, 1,4-butanediol, and 1,3-propanediol. {\textcopyright} 2013",

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author = "Eriksen, {Dawn T.} and Sijin Li and Huimin Zhao",

note = "Funding Information: We thank the National Institutes of Health (GM077596), the National Academies Keck Futures Initiative on Synthetic Biology, the Energy Biosciences Institute, and the National Science Foundation as part of the Center for Enabling New Technologies through Catalysis (CENTC), CHE-0650456 for financial support in our synthetic biology projects. Publisher Copyright: {\textcopyright} 2013 Elsevier Inc.",

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AU - Eriksen, Dawn T.

AU - Li, Sijin

AU - Zhao, Huimin

N1 - Funding Information: We thank the National Institutes of Health (GM077596), the National Academies Keck Futures Initiative on Synthetic Biology, the Energy Biosciences Institute, and the National Science Foundation as part of the Center for Enabling New Technologies through Catalysis (CENTC), CHE-0650456 for financial support in our synthetic biology projects. Publisher Copyright: © 2013 Elsevier Inc.

PY - 2013

Y1 - 2013

N2 - Microbial synthesis of value-added compounds often depends upon the creation or introduction of heterologous metabolic pathways into production hosts. However, there are many challenges in the engineering of a pathway and even more issues in optimizing it for the highest product yield. In this chapter, we will review a wide variety of computational and experimental tools that have been developed to design, construct, and optimize metabolic pathways. In addition, we will illustrate the applications of these tools in synthetic biology by highlighting the engineering of three metabolic pathways involved in the synthesis of glucaric acid, 1,4-butanediol, and 1,3-propanediol. © 2013

AB - Microbial synthesis of value-added compounds often depends upon the creation or introduction of heterologous metabolic pathways into production hosts. However, there are many challenges in the engineering of a pathway and even more issues in optimizing it for the highest product yield. In this chapter, we will review a wide variety of computational and experimental tools that have been developed to design, construct, and optimize metabolic pathways. In addition, we will illustrate the applications of these tools in synthetic biology by highlighting the engineering of three metabolic pathways involved in the synthesis of glucaric acid, 1,4-butanediol, and 1,3-propanediol. © 2013

KW - DNA assembly

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KW - Pathway optimization

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ER -

Pathway engineering as an enabling synthetic biology tool

Abstract

Keywords

ASJC Scopus subject areas

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