Pathway engineering as an enabling synthetic biology tool

Dawn T. Eriksen, Sijin Li, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Microbial synthesis of value-added compounds often depends upon the creation or introduction of heterologous metabolic pathways into production hosts. However, there are many challenges in the engineering of a pathway and even more issues in optimizing it for the highest product yield. In this chapter, we will review a wide variety of computational and experimental tools that have been developed to design, construct, and optimize metabolic pathways. In addition, we will illustrate the applications of these tools in synthetic biology by highlighting the engineering of three metabolic pathways involved in the synthesis of glucaric acid, 1,4-butanediol, and 1,3-propanediol. © 2013

Original languageEnglish (US)
Title of host publicationSynthetic Biology
PublisherElsevier Inc.
Pages43-61
Number of pages19
ISBN (Print)9780123944306
DOIs
StatePublished - 2013

Keywords

  • DNA assembly
  • Metabolic flux balancing
  • Pathway engineering
  • Pathway optimization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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