Pathologic characteristics of ovarian hemorrhagic polycyst in estrogen receptor-alpha (ERα) knockout mice and roles of erα in hemorrhagic polycyst

Hyun Jin Son, Joo Heon Kim, Hye Kyung Lee, Mee Ja Park, Dong Wook Kang, Che Myong Ko

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy causing anovulation in women of childbearing age. It has been well established that estrogen receptor-alpha knockout (ERαKO) mice display sevERαl pathologic ovarian phenotypes of PCOS. The aims of this study were to determine ovarian pathology in new ERαKO mice using a CreloxP approach and intrα-ovarian ERα function as regulating key aspects of PCOS. Methods: ERαKO mice, which were deficient in exon 3 of the ERα gene, were used. Immunohistochemical studies were done on ovaries of control and ERαKO mice using antibodies specific to ERα, ERβ, inhibin-α, and a-smooth muscle actin (SMA), as well as histochemical staining using Sudan black-B. Results: All ovaries of ERαKO mice were larger than control mouse ovaries and displayed a disrupted theca-interstitial tissue organization, multiple atretic follicles and multiple hemorrhagic cysts. None of the ERαKO mouse ovaries showed a corpus luteum. In addition, heavy deposition of Sudan black-B positive foamy cells was seen. The theca externa of preantral immature follicles and hemorrhagic cysts showed strong expression of α-SMA. Conclusions: ERαKO mice show hemorrhagic polycystic ovaries and hyperplasia of the theca externa. This study demonstrates that the ERα is the functional key to the pathogenesis of PCOS.

Original languageEnglish (US)
Pages (from-to)376-383
Number of pages8
JournalKorean Journal of Pathology
Volume44
Issue number4
DOIs
StatePublished - Aug 1 2010
Externally publishedYes

Keywords

  • Estrogen receptor-alpha knockout (ERαKO)
  • Hemorrhagic polycysts
  • Immunohistochemistry
  • Polycystic ovary syndrome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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