TY - JOUR
T1 - Pathogenic role of SEF14, SEF17, and SEF21 fimbriae in Salmonella enterica serovar enteritidis infection of chickens
AU - Rajashekara, Gireesh
AU - Munir, Shirin
AU - Alexeyev, Mikhail F.
AU - Halvorson, David A.
AU - Wells, Carol L.
AU - Nagaraja, Kakambi V.
PY - 2000/4
Y1 - 2000/4
N2 - Very little is known about the contribution of surface appendages of Salmonella enterica serovar Enteritidis to pathogenesis in chickens. This study was designed to clarify the role of SEF14, SEF17, and SEF21 fimbriae in serovar Enteritidis pathogenesis. Stable, single, defined sefA (SEF14), agfa (SEF17), and fimA (SEF21) insertionally inactivated fimbrial gene mutants of serovar Enteritidis were constructed. All mutant strains invaded Caco-2 and HT-29 enterocytes at levels similar to that of the wild type. Both mutant and wild-type strains were ingested equally well by chicken macrophage cell lines HD11 and MQ-NCSU. There were no significant differences in the abilities of these strains to colonize chicken ceca. The SEF14- strain was isolated in lower numbers from the livers of infected chickens and was cleared from the spleens faster than other strains. No significant differences in fecal shedding of these strains were observed.
AB - Very little is known about the contribution of surface appendages of Salmonella enterica serovar Enteritidis to pathogenesis in chickens. This study was designed to clarify the role of SEF14, SEF17, and SEF21 fimbriae in serovar Enteritidis pathogenesis. Stable, single, defined sefA (SEF14), agfa (SEF17), and fimA (SEF21) insertionally inactivated fimbrial gene mutants of serovar Enteritidis were constructed. All mutant strains invaded Caco-2 and HT-29 enterocytes at levels similar to that of the wild type. Both mutant and wild-type strains were ingested equally well by chicken macrophage cell lines HD11 and MQ-NCSU. There were no significant differences in the abilities of these strains to colonize chicken ceca. The SEF14- strain was isolated in lower numbers from the livers of infected chickens and was cleared from the spleens faster than other strains. No significant differences in fecal shedding of these strains were observed.
UR - https://www.scopus.com/pages/publications/0034074416
UR - https://www.scopus.com/inward/citedby.url?scp=0034074416&partnerID=8YFLogxK
U2 - 10.1128/AEM.66.4.1759-1763.2000
DO - 10.1128/AEM.66.4.1759-1763.2000
M3 - Article
C2 - 10742278
AN - SCOPUS:0034074416
SN - 0099-2240
VL - 66
SP - 1759
EP - 1763
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 4
ER -