TY - CHAP
T1 - Pasteurella multocida toxin
AU - Wilson, Brenda A.
AU - Ho, Mengfei
N1 - Funding Information:
Some of the work reported here was supported by grants from the National Institutes of Health (NIAID/AI38396) and from the United States Department of Agriculture (NRI/1999-02295) (to B.A.W.).
PY - 2006
Y1 - 2006
N2 - The major virulence factor of Pasteurella multocida responsible for atrophic rhinitis, pneumonia-like respiratory disease, and dermonecrosis is a monomeric 1285 amino acid protein toxin (PMT) produced primarily by capsular type D, some capsular type A, and a few non-typable strains. This chapter reviews what is currently known about the structure and molecular action of PMT, its role in pathogenesis, and its interaction with and effects on mammalian cells. Examples are also provided of the use of PMT as a powerful tool to study mitogenic, Gq-protein-, and Rho-protein-dependent signaling mechanisms. The understanding of PMT structure and activity is at a much earlier stage, and a number of outstanding questions remain about its functional organization, additional intracellular targets, and biochemical activity. Yet, because of its unique action on Gq, PMT is already being used as a potent research tool in deciphering cellular signal transduction pathways involving Gq-proteins. PMT facilitation of Gqα-protein coupling to PLCβ1 causes the same cellular responses elicited by Gq-protein-coupled receptors. Down-regulation of Gq- PLC signaling upon prolonged PMT treatment alsoallows for distinguishing between PTx-sensitive and PTx-insensitive signaling pathways that involve Gq.
AB - The major virulence factor of Pasteurella multocida responsible for atrophic rhinitis, pneumonia-like respiratory disease, and dermonecrosis is a monomeric 1285 amino acid protein toxin (PMT) produced primarily by capsular type D, some capsular type A, and a few non-typable strains. This chapter reviews what is currently known about the structure and molecular action of PMT, its role in pathogenesis, and its interaction with and effects on mammalian cells. Examples are also provided of the use of PMT as a powerful tool to study mitogenic, Gq-protein-, and Rho-protein-dependent signaling mechanisms. The understanding of PMT structure and activity is at a much earlier stage, and a number of outstanding questions remain about its functional organization, additional intracellular targets, and biochemical activity. Yet, because of its unique action on Gq, PMT is already being used as a potent research tool in deciphering cellular signal transduction pathways involving Gq-proteins. PMT facilitation of Gqα-protein coupling to PLCβ1 causes the same cellular responses elicited by Gq-protein-coupled receptors. Down-regulation of Gq- PLC signaling upon prolonged PMT treatment alsoallows for distinguishing between PTx-sensitive and PTx-insensitive signaling pathways that involve Gq.
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U2 - 10.1016/B978-012088445-2/50027-5
DO - 10.1016/B978-012088445-2/50027-5
M3 - Chapter
AN - SCOPUS:34548435506
SN - 9780120884452
SP - 430
EP - 447
BT - The Comprehensive Sourcebook of Bacterial Protein Toxins
PB - Elsevier Inc.
ER -