Abstract
Uridine diphosphate-D-galactose: inositol galactosyltransferase, the enzyme responsible for galactinol synthesis, was purified 41-fold from leaves of Cucumis sativus L. by a combination of gel filtration and ion exchange chromatography. Mn2+ concentration influenced the pH optimum of the enzyme. At low Mn2+ concentration (0.2 mM) the optimum was at pH 7.0. At high Mn2+ concentration (7.0 mM) the optimum was pH 5.5. The enzyme was not found in lysates of chloroplasts isolated from cucumber leaves.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 25-33 |
| Number of pages | 9 |
| Journal | Plant Science Letters |
| Volume | 23 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1981 |
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Pharr, D. M., Sox, H. N., Locy, R. D., & Huber, S. C. (1981). Partial characterization of the galactinol forming enzyme from leaves of cucumis sativus L. Plant Science Letters, 23(1), 25-33. https://doi.org/10.1016/0304-4211(81)90021-3