Abstract
Uridine diphosphate-D-galactose: inositol galactosyltransferase, the enzyme responsible for galactinol synthesis, was purified 41-fold from leaves of Cucumis sativus L. by a combination of gel filtration and ion exchange chromatography. Mn2+ concentration influenced the pH optimum of the enzyme. At low Mn2+ concentration (0.2 mM) the optimum was at pH 7.0. At high Mn2+ concentration (7.0 mM) the optimum was pH 5.5. The enzyme was not found in lysates of chloroplasts isolated from cucumber leaves.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 25-33 |
| Number of pages | 9 |
| Journal | Plant Science Letters |
| Volume | 23 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1981 |
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Partial characterization of the galactinol forming enzyme from leaves of cucumis sativus L. / Pharr, D. M.; Sox, H. N.; Locy, R. D.; Huber, Steven C.
In: Plant Science Letters, Vol. 23, No. 1, 10.1981, p. 25-33.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Partial characterization of the galactinol forming enzyme from leaves of cucumis sativus L.
AU - Pharr, D. M.
AU - Sox, H. N.
AU - Locy, R. D.
AU - Huber, Steven C
PY - 1981/10
Y1 - 1981/10
N2 - Uridine diphosphate-D-galactose: inositol galactosyltransferase, the enzyme responsible for galactinol synthesis, was purified 41-fold from leaves of Cucumis sativus L. by a combination of gel filtration and ion exchange chromatography. Mn2+ concentration influenced the pH optimum of the enzyme. At low Mn2+ concentration (0.2 mM) the optimum was at pH 7.0. At high Mn2+ concentration (7.0 mM) the optimum was pH 5.5. The enzyme was not found in lysates of chloroplasts isolated from cucumber leaves.
AB - Uridine diphosphate-D-galactose: inositol galactosyltransferase, the enzyme responsible for galactinol synthesis, was purified 41-fold from leaves of Cucumis sativus L. by a combination of gel filtration and ion exchange chromatography. Mn2+ concentration influenced the pH optimum of the enzyme. At low Mn2+ concentration (0.2 mM) the optimum was at pH 7.0. At high Mn2+ concentration (7.0 mM) the optimum was pH 5.5. The enzyme was not found in lysates of chloroplasts isolated from cucumber leaves.
UR - http://www.scopus.com/inward/record.url?scp=0342690544&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0342690544&partnerID=8YFLogxK
U2 - 10.1016/0304-4211(81)90021-3
DO - 10.1016/0304-4211(81)90021-3
M3 - Article
AN - SCOPUS:0342690544
VL - 23
SP - 25
EP - 33
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 1
ER -