Partial characterization of the galactinol forming enzyme from leaves of cucumis sativus L.

D. M. Pharr; H. N. Sox; R. D. Locy; Steven C Huber

doi:10.1016/0304-4211(81)90021-3

Partial characterization of the galactinol forming enzyme from leaves of cucumis sativus L.

D. M. Pharr, H. N. Sox, R. D. Locy, Steven C Huber

Plant Biology

Research output: Contribution to journal › Article

Abstract

Uridine diphosphate-D-galactose: inositol galactosyltransferase, the enzyme responsible for galactinol synthesis, was purified 41-fold from leaves of Cucumis sativus L. by a combination of gel filtration and ion exchange chromatography. Mn²⁺ concentration influenced the pH optimum of the enzyme. At low Mn²⁺ concentration (0.2 mM) the optimum was at pH 7.0. At high Mn²⁺ concentration (7.0 mM) the optimum was pH 5.5. The enzyme was not found in lysates of chloroplasts isolated from cucumber leaves.

Original language	English (US)
Pages (from-to)	25-33
Number of pages	9
Journal	Plant Science Letters
Volume	23
Issue number	1
DOIs	https://doi.org/10.1016/0304-4211(81)90021-3
State	Published - Oct 1981

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Pharr, D. M., Sox, H. N., Locy, R. D., & Huber, S. C. (1981). Partial characterization of the galactinol forming enzyme from leaves of cucumis sativus L. Plant Science Letters, 23(1), 25-33. https://doi.org/10.1016/0304-4211(81)90021-3

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10.1016/0304-4211(81)90021-3