Partial characterization of the galactinol forming enzyme from leaves of cucumis sativus L.

D. M. Pharr, H. N. Sox, R. D. Locy, Steven C Huber

Research output: Contribution to journalArticle


Uridine diphosphate-D-galactose: inositol galactosyltransferase, the enzyme responsible for galactinol synthesis, was purified 41-fold from leaves of Cucumis sativus L. by a combination of gel filtration and ion exchange chromatography. Mn2+ concentration influenced the pH optimum of the enzyme. At low Mn2+ concentration (0.2 mM) the optimum was at pH 7.0. At high Mn2+ concentration (7.0 mM) the optimum was pH 5.5. The enzyme was not found in lysates of chloroplasts isolated from cucumber leaves.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalPlant Science Letters
Issue number1
StatePublished - Oct 1981


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