Papaya CpbHLH1/2 regulate carotenoid biosynthesis-related genes during papaya fruit ripening

Dong Zhou, Yanhong Shen, Ping Zhou, Mahpara Fatima, Jishan Lin, Jingjing Yue, Xingtan Zhang, Li Yu Chen, Ray R Ming

Research output: Contribution to journalArticle

Abstract

The ripening of papaya is a physiological and metabolic process associated with accumulation of carotenoids, alternation of flesh color and flavor, which depending on genotype and external factors such as light and hormone. Transcription factors regulating carotenoid biosynthesis have not been analyzed during papaya fruit ripening. RNA-Seq experiments were implemented using different ripening stages of papaya fruit from two papaya varieties. Cis-elements in lycopene β-cyclase genes (CpCYC-B and CpLCY-B) were identified, and followed by genome-wide analysis to identify transcription factors binding to these cis-elements, resulting in the identification of CpbHLH1 and CpbHLH2, two bHLH genes. The expressions of CpbHLH1/2 were changed during fruit development, coupled with transcript increase of carotenoid biosynthesis-related genes including CpCYC-B, CpLCY-B, CpPDS2, CpZDS, CpLCY-E, and CpCHY-B. Yeast one-hybrid (Y1H) and transient expression assay revealed that CpbHLH1/2 could bind to the promoters of CpCYC-B and CpLCY-B, and regulate their transcriptions. In response to strong light, the results of elevated expression of carotenoid biosynthesis-related genes and the changed expression of CpbHLH1/2 indicated that CpbHLH1/2 were involved in light-mediated mechanisms of regulating critical genes in the carotenoid biosynthesis pathway. Collectively, our findings demonstrated several TF family members participating in the regulation of carotenoid genes and proved that CpbHLH1 and CpbHLH2 individually regulated the transcription of lycopene β-cyclase genes (CpCYC-B and CpLCY-B). This study yielded novel findings on regulatory mechanism of carotenoid biosynthesis during papaya fruit ripening.

Original languageEnglish (US)
Article number80
JournalHorticulture Research
Volume6
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

Carica
Biosynthesis
papayas
Carotenoids
Fruits
Fruit
carotenoids
ripening
Genes
biosynthesis
fruits
genes
lycopene
Light
Transcription
Transcription Factors
transcription factors
transcription (genetics)
Physiological Phenomena
Flavors

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Genetics
  • Plant Science
  • Horticulture

Cite this

Papaya CpbHLH1/2 regulate carotenoid biosynthesis-related genes during papaya fruit ripening. / Zhou, Dong; Shen, Yanhong; Zhou, Ping; Fatima, Mahpara; Lin, Jishan; Yue, Jingjing; Zhang, Xingtan; Chen, Li Yu; Ming, Ray R.

In: Horticulture Research, Vol. 6, No. 1, 80, 01.12.2019.

Research output: Contribution to journalArticle

Zhou, Dong ; Shen, Yanhong ; Zhou, Ping ; Fatima, Mahpara ; Lin, Jishan ; Yue, Jingjing ; Zhang, Xingtan ; Chen, Li Yu ; Ming, Ray R. / Papaya CpbHLH1/2 regulate carotenoid biosynthesis-related genes during papaya fruit ripening. In: Horticulture Research. 2019 ; Vol. 6, No. 1.
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abstract = "The ripening of papaya is a physiological and metabolic process associated with accumulation of carotenoids, alternation of flesh color and flavor, which depending on genotype and external factors such as light and hormone. Transcription factors regulating carotenoid biosynthesis have not been analyzed during papaya fruit ripening. RNA-Seq experiments were implemented using different ripening stages of papaya fruit from two papaya varieties. Cis-elements in lycopene β-cyclase genes (CpCYC-B and CpLCY-B) were identified, and followed by genome-wide analysis to identify transcription factors binding to these cis-elements, resulting in the identification of CpbHLH1 and CpbHLH2, two bHLH genes. The expressions of CpbHLH1/2 were changed during fruit development, coupled with transcript increase of carotenoid biosynthesis-related genes including CpCYC-B, CpLCY-B, CpPDS2, CpZDS, CpLCY-E, and CpCHY-B. Yeast one-hybrid (Y1H) and transient expression assay revealed that CpbHLH1/2 could bind to the promoters of CpCYC-B and CpLCY-B, and regulate their transcriptions. In response to strong light, the results of elevated expression of carotenoid biosynthesis-related genes and the changed expression of CpbHLH1/2 indicated that CpbHLH1/2 were involved in light-mediated mechanisms of regulating critical genes in the carotenoid biosynthesis pathway. Collectively, our findings demonstrated several TF family members participating in the regulation of carotenoid genes and proved that CpbHLH1 and CpbHLH2 individually regulated the transcription of lycopene β-cyclase genes (CpCYC-B and CpLCY-B). This study yielded novel findings on regulatory mechanism of carotenoid biosynthesis during papaya fruit ripening.",
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AU - Zhang, Xingtan

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