Oxygen Nanobubble Tracking by Light Scattering in Single Cells and Tissues

Pushpak Bhandari, Xiaolei Wang, Joseph Irudayaraj

Research output: Contribution to journalArticlepeer-review


Oxygen nanobubbles (ONBs) have significant potential in targeted imaging and treatment in cancer diagnosis and therapy. Precise localization and tracking of single ONBs is demonstrated based on hyperspectral dark-field microscope (HSDFM) to image and track single oxygen nanobubbles in single cells. ONBs were proposed as promising contrast-generating imaging agents due to their strong light scattering generated from nonuniformity of refractive index at the interface. With this powerful platform, we have revealed the trajectories and quantities of ONBs in cells, and demonstrated the relation between the size and diffusion coefficient. We have also evaluated the presence of ONBs in the nucleus with respect to an increase in incubation time and have quantified the uptake in single cells in ex vivo tumor tissues. Our results demonstrate that HSDFM can be a versatile platform to detect and measure cellulosic nanoparticles at the single-cell level and to assess the dynamics and trajectories of this delivery system.

Original languageEnglish (US)
Pages (from-to)2682-2688
Number of pages7
JournalACS Nano
Issue number3
StatePublished - Mar 28 2017
Externally publishedYes


  • diffusion
  • hyperspectral dark-field microscope
  • oxygen nanobubbles
  • quantification
  • single cell analysis
  • tracking

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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