Oxidative phosphorylation has been demonstrated in mitochondria isolated from Euglena gracilis with a variety of substrates including NADH2, but not NADPH2. Both oxidation and phosphorylation were inhibited by DNP and cyanide with phosphorylation being the more sensitive. The effects of amytal, rotenone and anti-mycin A parallel their effects in mammalian mitochondria with retenone the most inhibitory. The measured P:O ratios showed one less phosphorylation per μatom oxygen than usually observed in mammalian mitochondria. The results suggest the absence, or the presence in limiting amount, of some component in the electron transport chain of these mitochondria.
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