Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates

R. Louis Schiltz, Craig Andrew Mizzen, Alex Vassilev, Richard G. Cook, C. David Allis, Yoshihiro Nakatani

Research output: Contribution to journalArticle

Abstract

A number of transcriptional coactivators possess intrinsic histone acetylase activity, providing a direct link between hyperacetylated chromatin and transcriptional activation. We have determined the core histone residues acetylated in vitro by recombinant p300 and PCAF within mononucleosomes. p300 specifically acetylates all sites of histones H2A and H2B known to be acetylated in bulk chromatin in vivo but preferentially acetylates lysines 14 and 18 of histone H3 and lysines 5 and 8 of histone H4. PCAF primarily acetylates lysine 14 of H3 but also less efficiently acetylates lysine 8 of H4. PCAF in its native form, which is present in a stable multimeric protein complex lacking p300/CBP, primarily acetylates H3 to a monoacetylated form, suggesting that PCAF-associated polypeptides do not alter the substrate specificity. These distinct patterns of acetylation by the p300 and PCAF may contribute to their differential roles in transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)1189-1192
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number3
DOIs
StatePublished - Jan 15 1999

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Acetylation
Histones
Lysine
Substrates
Chromatin
Histone Acetyltransferases
Substrate Specificity
Transcriptional Activation
Chemical activation
Peptides
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates. / Schiltz, R. Louis; Mizzen, Craig Andrew; Vassilev, Alex; Cook, Richard G.; Allis, C. David; Nakatani, Yoshihiro.

In: Journal of Biological Chemistry, Vol. 274, No. 3, 15.01.1999, p. 1189-1192.

Research output: Contribution to journalArticle

Schiltz, R. Louis ; Mizzen, Craig Andrew ; Vassilev, Alex ; Cook, Richard G. ; Allis, C. David ; Nakatani, Yoshihiro. / Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 3. pp. 1189-1192.
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