Overcoming steric hindrances during replication of peripheral heterochromatin

O. A. Zhironkina, S. Yu Kurchashova, A. L. Brattseva, V. D. Cherepaninets, O. S. Strelkova, A. S. Belmont, I. I. Kireev

Research output: Contribution to journalArticle

Abstract

Due to a tight attachment of peripheral heterochromatin to the nuclear lamina its replication is connected with inevitable topological hindrances. Additional hindrances are caused by high stability of the lamina that complicates the access of replication factors to DNA duplication sites under conditions of highly condensed matrix with limited mobility. The work focuses on detailed study of structural organization and dynamics of the lamina in respect to replication of peripheral heterochromatin that is attached to it. The study of mobile properties of lamins at various stages of the S-phase using live cell imaging and super-resolution microscopy showed the absence of the dependence of lamins’ mobility on replicative status of attached heterochromatin. These data confirm the hypothesis on regulation of linkage between chromatin and lamina at the level of molecular intermediates. It has been shown at the ultrastructural level that possible temporary disruption in molecular bonds between the lamina and peripheral chromatin during replication does not cause movement of replicated domains from the nuclear periphery.

Original languageEnglish (US)
Pages (from-to)110-118
Number of pages9
JournalCell and Tissue Biology
Volume9
Issue number2
DOIs
StatePublished - Mar 1 2015

Fingerprint

Heterochromatin
Lamins
Chromatin
Nuclear Lamina
S Phase
Microscopy
DNA

Keywords

  • FRAP
  • electron microscopy
  • lamina
  • nuclear envelope
  • replication
  • structured illumination microscopy

ASJC Scopus subject areas

  • Cell Biology

Cite this

Zhironkina, O. A., Kurchashova, S. Y., Brattseva, A. L., Cherepaninets, V. D., Strelkova, O. S., Belmont, A. S., & Kireev, I. I. (2015). Overcoming steric hindrances during replication of peripheral heterochromatin. Cell and Tissue Biology, 9(2), 110-118. https://doi.org/10.1134/S1990519X15020121

Overcoming steric hindrances during replication of peripheral heterochromatin. / Zhironkina, O. A.; Kurchashova, S. Yu; Brattseva, A. L.; Cherepaninets, V. D.; Strelkova, O. S.; Belmont, A. S.; Kireev, I. I.

In: Cell and Tissue Biology, Vol. 9, No. 2, 01.03.2015, p. 110-118.

Research output: Contribution to journalArticle

Zhironkina, OA, Kurchashova, SY, Brattseva, AL, Cherepaninets, VD, Strelkova, OS, Belmont, AS & Kireev, II 2015, 'Overcoming steric hindrances during replication of peripheral heterochromatin', Cell and Tissue Biology, vol. 9, no. 2, pp. 110-118. https://doi.org/10.1134/S1990519X15020121
Zhironkina OA, Kurchashova SY, Brattseva AL, Cherepaninets VD, Strelkova OS, Belmont AS et al. Overcoming steric hindrances during replication of peripheral heterochromatin. Cell and Tissue Biology. 2015 Mar 1;9(2):110-118. https://doi.org/10.1134/S1990519X15020121
Zhironkina, O. A. ; Kurchashova, S. Yu ; Brattseva, A. L. ; Cherepaninets, V. D. ; Strelkova, O. S. ; Belmont, A. S. ; Kireev, I. I. / Overcoming steric hindrances during replication of peripheral heterochromatin. In: Cell and Tissue Biology. 2015 ; Vol. 9, No. 2. pp. 110-118.
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