TY - JOUR
T1 - Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance
AU - He, Chao
AU - Liang, Jinjin
AU - Yang, Jing
AU - Xue, Hu
AU - Huang, Mingjiao
AU - Fu, Buli
AU - Wei, Xuegao
AU - Liu, Shaonan
AU - Du, Tianhua
AU - Ji, Yao
AU - Yin, Cheng
AU - Gong, Peipan
AU - Hu, Jin Yu
AU - Du, He
AU - Zhang, Rong
AU - Xie, Wen
AU - Wang, Shaoli
AU - Wu, Qingjun
AU - Zhou, Xuguo
AU - Yang, Xin
AU - Zhang, Youjun
N1 - Publisher Copyright:
© 2023
PY - 2023/4/1
Y1 - 2023/4/1
N2 - Cuticular proteins (CPs) play an important role in protecting insects from adverse environmental conditions, like neonicotinoid insecticides, which are heavily used for numerous pests and caused environmental problems and public health concerns worldwide. However, the relationship between CPs and insecticides resistance in Bemisia tabaci, a serious and developed high insecticide resistance, is lacking. In this study, 125 CPs genes were identified in B. tabaci. Further phylogenetic tree showed the RR-2-type genes formed large gene groups in B. tabaci. Transcriptional expression levels of CPs genes at different developmental stages revealed that some CPs genes may play a specific role in insect development. The TEM results indicated that the cuticle thickness of susceptible strain was thinner than imidacloprid-resistance strain. Furthermore, 16 CPs genes (5 in RR-1 subfamily, 7 in RR-2 subfamily, 3 in CPAP3 subfamily and 1 in CPCFC subfamily) were activated in response to imidacloprid. And RNAi results indicated that CP9 and CP83 involved in imidacloprid resistance. In conclusion, this study was the first time to establish a basic information framework and evolutionary relationship between CPs and imidacloprid resistance in B. tabaci, which provides a basis for proposing integrated pest management strategies.
AB - Cuticular proteins (CPs) play an important role in protecting insects from adverse environmental conditions, like neonicotinoid insecticides, which are heavily used for numerous pests and caused environmental problems and public health concerns worldwide. However, the relationship between CPs and insecticides resistance in Bemisia tabaci, a serious and developed high insecticide resistance, is lacking. In this study, 125 CPs genes were identified in B. tabaci. Further phylogenetic tree showed the RR-2-type genes formed large gene groups in B. tabaci. Transcriptional expression levels of CPs genes at different developmental stages revealed that some CPs genes may play a specific role in insect development. The TEM results indicated that the cuticle thickness of susceptible strain was thinner than imidacloprid-resistance strain. Furthermore, 16 CPs genes (5 in RR-1 subfamily, 7 in RR-2 subfamily, 3 in CPAP3 subfamily and 1 in CPCFC subfamily) were activated in response to imidacloprid. And RNAi results indicated that CP9 and CP83 involved in imidacloprid resistance. In conclusion, this study was the first time to establish a basic information framework and evolutionary relationship between CPs and imidacloprid resistance in B. tabaci, which provides a basis for proposing integrated pest management strategies.
KW - Bemisia tabaci
KW - Cuticular proteins
KW - Expression patterns
KW - Imidacloprid
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U2 - 10.1016/j.ijbiomac.2023.123647
DO - 10.1016/j.ijbiomac.2023.123647
M3 - Article
C2 - 36780959
AN - SCOPUS:85147932538
SN - 0141-8130
VL - 233
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 123647
ER -