Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance

Chao He, Jinjin Liang, Jing Yang, Hu Xue, Mingjiao Huang, Buli Fu, Xuegao Wei, Shaonan Liu, Tianhua Du, Yao Ji, Cheng Yin, Peipan Gong, Jin Yu Hu, He Du, Rong Zhang, Wen Xie, Shaoli Wang, Qingjun Wu, Xuguo Zhou, Xin YangYoujun Zhang

Research output: Contribution to journalArticlepeer-review


Cuticular proteins (CPs) play an important role in protecting insects from adverse environmental conditions, like neonicotinoid insecticides, which are heavily used for numerous pests and caused environmental problems and public health concerns worldwide. However, the relationship between CPs and insecticides resistance in Bemisia tabaci, a serious and developed high insecticide resistance, is lacking. In this study, 125 CPs genes were identified in B. tabaci. Further phylogenetic tree showed the RR-2-type genes formed large gene groups in B. tabaci. Transcriptional expression levels of CPs genes at different developmental stages revealed that some CPs genes may play a specific role in insect development. The TEM results indicated that the cuticle thickness of susceptible strain was thinner than imidacloprid-resistance strain. Furthermore, 16 CPs genes (5 in RR-1 subfamily, 7 in RR-2 subfamily, 3 in CPAP3 subfamily and 1 in CPCFC subfamily) were activated in response to imidacloprid. And RNAi results indicated that CP9 and CP83 involved in imidacloprid resistance. In conclusion, this study was the first time to establish a basic information framework and evolutionary relationship between CPs and imidacloprid resistance in B. tabaci, which provides a basis for proposing integrated pest management strategies.

Original languageEnglish (US)
Article number123647
JournalInternational Journal of Biological Macromolecules
StatePublished - Apr 1 2023
Externally publishedYes


  • Bemisia tabaci
  • Cuticular proteins
  • Expression patterns
  • Imidacloprid

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology


Dive into the research topics of 'Over-expression of CP9 and CP83 increases whitefly cell cuticle thickness leading to imidacloprid resistance'. Together they form a unique fingerprint.

Cite this