TY - JOUR
T1 - Ottowia shaoguanensis sp. nov., Isolated from coking wastewater
AU - Geng, Shuang
AU - Pan, Xin Chi
AU - Mei, Ran
AU - Wang, Ya Nan
AU - Sun, Ji Quan
AU - Liu, Xue Ying
AU - Tang, Yue Qin
AU - Wu, Xiao Lei
N1 - The authors thank the Institute of Microbiology, Chinese Academy of Sciences, for the help in transmission electron microscopy analysis. This study was supported by National Natural Science Foundation of China (31070107 and 31225001), and the National High Technology Research and Development Program (“863” Program: 2012AA02A703).
PY - 2014/3
Y1 - 2014/3
N2 - A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66 T without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 C, and 1-2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699T. The major cellular fatty acids of strain J5-66 T were C16:1 ω7c/C16:1 ω6c (45.0 %), C16:0 (21.1 %), C18:1 ω7c or/and C 18:1 ω6c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66T represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66T (=CGMCC 1.12431T =LMG 27408T).
AB - A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66 T without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 C, and 1-2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699T. The major cellular fatty acids of strain J5-66 T were C16:1 ω7c/C16:1 ω6c (45.0 %), C16:0 (21.1 %), C18:1 ω7c or/and C 18:1 ω6c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66T represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66T (=CGMCC 1.12431T =LMG 27408T).
UR - https://www.scopus.com/pages/publications/84893899253
UR - https://www.scopus.com/pages/publications/84893899253#tab=citedBy
U2 - 10.1007/s00284-013-0481-8
DO - 10.1007/s00284-013-0481-8
M3 - Article
C2 - 24158461
AN - SCOPUS:84893899253
SN - 0343-8651
VL - 68
SP - 324
EP - 329
JO - Current Microbiology
JF - Current Microbiology
IS - 3
ER -