Ottowia shaoguanensis sp. nov., Isolated from coking wastewater

Shuang Geng, Xin Chi Pan, Ran Mei, Ya Nan Wang, Ji Quan Sun, Xue Ying Liu, Yue Qin Tang, Xiao Lei Wu

Research output: Contribution to journalArticlepeer-review

Abstract

A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66 T without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 C, and 1-2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699T. The major cellular fatty acids of strain J5-66 T were C16:1 ω7c/C16:1 ω6c (45.0 %), C16:0 (21.1 %), C18:1 ω7c or/and C 18:1 ω6c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66T represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66T (=CGMCC 1.12431T =LMG 27408T).

Original languageEnglish (US)
Pages (from-to)324-329
Number of pages6
JournalCurrent Microbiology
Volume68
Issue number3
DOIs
StatePublished - Mar 2014
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Ottowia shaoguanensis sp. nov., Isolated from coking wastewater'. Together they form a unique fingerprint.

Cite this