TY - JOUR
T1 - Ottowia shaoguanensis sp. nov., Isolated from coking wastewater
AU - Geng, Shuang
AU - Pan, Xin Chi
AU - Mei, Ran
AU - Wang, Ya Nan
AU - Sun, Ji Quan
AU - Liu, Xue Ying
AU - Tang, Yue Qin
AU - Wu, Xiao Lei
PY - 2014/3
Y1 - 2014/3
N2 - A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66 T without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 C, and 1-2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699T. The major cellular fatty acids of strain J5-66 T were C16:1 ω7c/C16:1 ω6c (45.0 %), C16:0 (21.1 %), C18:1 ω7c or/and C 18:1 ω6c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66T represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66T (=CGMCC 1.12431T =LMG 27408T).
AB - A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66 T without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 C, and 1-2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699T. The major cellular fatty acids of strain J5-66 T were C16:1 ω7c/C16:1 ω6c (45.0 %), C16:0 (21.1 %), C18:1 ω7c or/and C 18:1 ω6c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66T represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66T (=CGMCC 1.12431T =LMG 27408T).
UR - http://www.scopus.com/inward/record.url?scp=84893899253&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893899253&partnerID=8YFLogxK
U2 - 10.1007/s00284-013-0481-8
DO - 10.1007/s00284-013-0481-8
M3 - Article
C2 - 24158461
AN - SCOPUS:84893899253
SN - 0343-8651
VL - 68
SP - 324
EP - 329
JO - Current Microbiology
JF - Current Microbiology
IS - 3
ER -