Orientation-selected 15N-HYSCORE detection of weakly coupled nitrogens around the archaeal rieske [2Fe-2S] center

Toshio Iwasaki, Asako Kounosu, Taketoshi Uzawa, Rimma I. Samoilova, Sergei A. Dikanov

Research output: Contribution to journalArticlepeer-review

Abstract

The weakly coupled 15N atoms around a reduced Rieske [2Fe-2S] cluster of the uniformly 15N-labeled, hyperthermostable archaeal Rieske protein appear to produce readily observable cross-peaks in the HYSCORE spectra, with the well-resolved couplings of 0.3-0.4 MHz for the Nε and 1.1 MHz for the peptide backbone nitrogens, in addition to the contributions from the coordinated Nδ atoms. These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly coupled nitrogens in coupled electron-proton transfer reactions.

Original languageEnglish (US)
Pages (from-to)13902-13903
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number43
DOIs
StatePublished - Nov 3 2004
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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