Orchardgrass (Dactylis glomerata L.) EST and SSR marker development, annotation, and transferability

B. Shaun Bushman, Steve R. Larson, Metin Tuna, Mark S. West, Alvaro G. Hernandez, Deepika Vullaganti, George Gong, Joseph G. Robins, Kevin B. Jensen, Jyothi Thimmapuram

Research output: Contribution to journalArticlepeer-review

Abstract

Orchardgrass, or cocksfoot [Dactylis glomerata (L.)], has been naturalized on nearly every continent and is a commonly used species for forage and hay production. All major cultivated varieties of orchardgrass are autotetraploid, and few tools or information are available for functional and comparative genetic analyses and improvement of the species. To improve the genetic resources for orchardgrass, we have developed an EST library and SSR markers from salt, drought, and cold stressed tissues. The ESTs were bi-directionally sequenced from clones and combined into 17,373 unigenes. Unigenes were annotated based on putative orthology to genes from rice, Triticeae grasses, other Poaceae, Arabidopsis, and the non-redundant database of the NCBI. Of 1,162 SSR markers developed, approximately 80% showed amplification products across a set of orchardgrass germplasm, and 40% across related Festuca and Lolium species. When orchardgrass subspecies were genotyped using 33 SSR markers their within-accession similarity values ranged from 0.44 to 0.71, with Mediterranean accessions having a higher similarity. The total number of genotyped bands was greater for tetraploid accessions compared to diploid accessions. Clustering analysis indicated grouping of Mediterranean subspecies and central Asian subspecies, while the D. glomerata ssp. aschersoniana was closest related to three cultivated varieties.

Original languageEnglish (US)
Pages (from-to)119-129
Number of pages11
JournalTheoretical and Applied Genetics
Volume123
Issue number1
DOIs
StatePublished - Jun 2011

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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