Optomechanical measurement of the stiffness of single adherent cells

Kidong Park, Ali Mehrnezhad, Elise A. Corbin, Rashid Bashir

Research output: Contribution to journalArticle

Abstract

Recent advances in mechanobiology have accumulated strong evidence showing close correlations between the physiological conditions and mechanical properties of cells. In this paper, a novel optomechanical technique to characterize the stiffness of single adherent cells attached on a substrate is reported. The oscillation in a cell's height on a vertically vibrating reflective substrate is measured with a laser Doppler vibrometer as apparent changes in the phase of the measured velocity. This apparent phase shift and the height oscillation are shown to be affected by the mechanical properties of human colorectal adenocarcinoma cells (HT-29). The reported optomechanical technique can provide high-throughput stiffness measurement of single adherent cells over time with minimal perturbation.

Original languageEnglish (US)
Pages (from-to)3460-3464
Number of pages5
JournalLab on a chip
Volume15
Issue number17
DOIs
StatePublished - Jul 14 2015

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ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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