Optimization of yeast and enzyme dose for dry grind ethanol plants

Samarth S.S. Rathore, Marvin R. Paulsen, Vivek Sharma, Vijay Singh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The objectives of this study were to determine the effect of enzyme and yeast dose on starch-to- glucose and glucose-to-ethanol conversions for the production of ethanol and sugars during the simultaneous saccharification and fermentation (SSF) process used in the dry grind ethanol industry. Fermentations were performed on a yellow dent corn hybrid with 3 enzymes doses (80, 110, and 140 μL) of spirilizyme glucoamylase enzyme and 3 yeast doses (2, 4, and 6 ml of 0.1 g/mL of yeast water slurry) with 3 replicates. Samples were drawn after 2, 4, 6, 8, 10, 12, 24, 48, and 72 hours from the start of the fermentation. Samples were analyzed using HPLC and FT-NIR spectroscopy for the ethanol and sugar contents. Enzyme and yeast doses both had significant effects on the level of sugar present during the SSF process. However, only yeast dose significantly affected ethanol content. Measuring enzyme and yeast dose using NIR provides a dynamic way to optimize the process.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008
PublisherAmerican Society of Agricultural and Biological Engineers
Pages2503-2516
Number of pages14
ISBN (Print)9781605605364
StatePublished - 2008
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008 - Providence, RI, United States
Duration: Jun 29 2008Jul 2 2008

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008
Volume4

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008
Country/TerritoryUnited States
CityProvidence, RI
Period6/29/087/2/08

Keywords

  • Dry grind ethanol
  • Enzyme
  • Near-infrared spectroscopy
  • Optimization
  • Response surface methodology
  • Yeast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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