TY - JOUR
T1 - Optimization of two-stage pretreatment for maximizing ethanol production in 1.5G technology
AU - Juneja, Ankita
AU - Noordam, Bert
AU - Pel, Herman
AU - Basu, Rahul
AU - Appeldoorn, Maaike
AU - Singh, Vijay
N1 - Funding Information:
We would like to acknowledge DSM Biobased Products & Services (Elgin, IL, US) for funding support and donation of cellulases enzyme.
Publisher Copyright:
© 2020
PY - 2021/1
Y1 - 2021/1
N2 - Two-stage pretreatment conditions were optimized to convert corn fiber, separated from whole stillage in a corn dry grind ethanol plant, to fermentable sugars via hydrolysis. Liquid hot water pretreatment (25% solids) at 180 °C for 10 min, followed by three cycles of disk milling, provided maximum glucose, xylose, and arabinose yields of 88.5%, 41.0%, and 30.4% respectively after hydrolysis with Cellulase I. The glucose, xylose, and arabinose yields with Cellulase II at optimum conditions were 94.9%, 74.2%, and 66.3%, respectively. SSF of corn fiber using engineered yeast, with both Cellulase I and II, provided maximum ethanol concentrations of 2.13% and 2.73% (v/v). The protein content in the residual solid after fermentation was 47.95% and 52.05% for Cellulase I and II, respectively. This technology provides additional ethanol in a dry grind plant by converting corn fiber into ethanol and increases the protein content of DDGS, thereby improving the quality.
AB - Two-stage pretreatment conditions were optimized to convert corn fiber, separated from whole stillage in a corn dry grind ethanol plant, to fermentable sugars via hydrolysis. Liquid hot water pretreatment (25% solids) at 180 °C for 10 min, followed by three cycles of disk milling, provided maximum glucose, xylose, and arabinose yields of 88.5%, 41.0%, and 30.4% respectively after hydrolysis with Cellulase I. The glucose, xylose, and arabinose yields with Cellulase II at optimum conditions were 94.9%, 74.2%, and 66.3%, respectively. SSF of corn fiber using engineered yeast, with both Cellulase I and II, provided maximum ethanol concentrations of 2.13% and 2.73% (v/v). The protein content in the residual solid after fermentation was 47.95% and 52.05% for Cellulase I and II, respectively. This technology provides additional ethanol in a dry grind plant by converting corn fiber into ethanol and increases the protein content of DDGS, thereby improving the quality.
KW - 1.5G technology
KW - Corn fiber
KW - Ethanol
KW - Fermentation
KW - Hydrolysis
KW - Pretreatment
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U2 - 10.1016/j.biortech.2020.124380
DO - 10.1016/j.biortech.2020.124380
M3 - Article
C2 - 33217695
AN - SCOPUS:85096718784
SN - 0960-8524
VL - 320
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 124380
ER -