TY - JOUR
T1 - Optimization of Spectral and Spatial Conditions to Improve Super-Resolution Imaging of Plasmonic Nanoparticles
AU - De Silva Indrasekara, A. Swarnapali
AU - Shuang, Bo
AU - Hollenhorst, Franziska
AU - Hoener, Benjamin S.
AU - Hoggard, Anneli
AU - Chen, Sishan
AU - Villarreal, Eduardo
AU - Cai, Yi Yu
AU - Kisley, Lydia
AU - Derry, Paul J.
AU - Chang, Wei Shun
AU - Zubarev, Eugene R.
AU - Ringe, Emilie
AU - Link, Stephan
AU - Landes, Christy F.
N1 - The authors acknowledge funding from the National Science Foundation (Grant CHE-1151647 to C.F.L.; Grant CBET-1438634 to S.L. and C.F.L.; Grant DMR-1105878 to E.R.Z.), the Welch Foundation (Grants C-1787 to C.F.L. and C-1664 to S.L.), the Air Force Office of Scientific Research (MURI FA9550-15-1-0022 to S.L.), and the American Chemical Society Petroleum Research Fund (54684-ND5 to C.F.L. and 56256-DN15 to E.R.). A.H., L.K., and E.V. acknowledge the National Science Foundation Graduate Research Fellowship (Grant 0940902).
PY - 2017/1/5
Y1 - 2017/1/5
N2 - Interactions between fluorophores and plasmonic nanoparticles modify the fluorescence intensity, shape, and position of the observed emission pattern, thus inhibiting efforts to optically super-resolve plasmonic nanoparticles. Herein, we investigate the accuracy of localizing dye fluorescence as a function of the spectral and spatial separations between fluorophores (Alexa 647) and gold nanorods (NRs). The distance at which Alexa 647 interacts with NRs is varied by layer-by-layer polyelectrolyte deposition while the spectral separation is tuned by using NRs with varying localized surface plasmon resonance (LSPR) maxima. For resonantly coupled Alexa 647 and NRs, emission to the far field through the NR plasmon is highly prominent, resulting in underestimation of NR sizes. However, we demonstrate that it is possible to improve the accuracy of the emission localization when both the spectral and spatial separations between Alexa 647 and the LSPR are optimized.
AB - Interactions between fluorophores and plasmonic nanoparticles modify the fluorescence intensity, shape, and position of the observed emission pattern, thus inhibiting efforts to optically super-resolve plasmonic nanoparticles. Herein, we investigate the accuracy of localizing dye fluorescence as a function of the spectral and spatial separations between fluorophores (Alexa 647) and gold nanorods (NRs). The distance at which Alexa 647 interacts with NRs is varied by layer-by-layer polyelectrolyte deposition while the spectral separation is tuned by using NRs with varying localized surface plasmon resonance (LSPR) maxima. For resonantly coupled Alexa 647 and NRs, emission to the far field through the NR plasmon is highly prominent, resulting in underestimation of NR sizes. However, we demonstrate that it is possible to improve the accuracy of the emission localization when both the spectral and spatial separations between Alexa 647 and the LSPR are optimized.
UR - https://www.scopus.com/pages/publications/85018484587
UR - https://www.scopus.com/pages/publications/85018484587#tab=citedBy
U2 - 10.1021/acs.jpclett.6b02569
DO - 10.1021/acs.jpclett.6b02569
M3 - Article
C2 - 27982600
AN - SCOPUS:85018484587
SN - 1948-7185
VL - 8
SP - 299
EP - 306
JO - Journal of Physical Chemistry Letters
JF - Journal of Physical Chemistry Letters
IS - 1
ER -