Optimization of photonic crystal enhanced fluorescence by excitation laser angle scanning

Vikram Chaudhery, Meng Lu, Anusha Pokhriyal, Stephen C. Schulz, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photonic crystal enhanced fluorescence (PCEF) has been demonstrated as an effective technique for amplifying the electromagnetic excitation and emission extraction from surface-bound fluorescent molecules. Although optimal coupling of a fluorophore-exciting light source to the PC occurs with the use of collimated plane waves, PCEF surfaces are also capable of coupling light from focused sources but with a reduction in the obtainable enhancement factor. Using computer simulations and experimental measurements, we describe the interaction between the resonant bandwidth of a PCEF device surface and the optical design of the detection instrumentation that is used to provide fluorescence excitation. We show that highly collimated illumination is required for achieving the greatest PCEF enhancement factors, but at the expense of poor tolerance to non-uniformities in resonant wavelength across the PCEF surface. To overcome this limitation, we demonstrate a fixed wavelength/multiple incident angle scanning detection system that is capable of measuring every pixel in a PCEF fluorescence image under conditions that optimize resonant excitation efficiency.

Original languageEnglish (US)
Title of host publication2011 11th IEEE International Conference on Nanotechnology, NANO 2011
Pages282-285
Number of pages4
DOIs
StatePublished - Dec 1 2011
Event2011 11th IEEE International Conference on Nanotechnology, NANO 2011 - Portland, OR, United States
Duration: Aug 15 2011Aug 19 2011

Publication series

NameProceedings of the IEEE Conference on Nanotechnology
ISSN (Print)1944-9399
ISSN (Electronic)1944-9380

Other

Other2011 11th IEEE International Conference on Nanotechnology, NANO 2011
CountryUnited States
CityPortland, OR
Period8/15/118/19/11

Fingerprint

Laser excitation
Photonic crystals
Fluorescence
photonics
Scanning
fluorescence
optimization
scanning
excitation
crystals
lasers
Wavelength
Optical design
Fluorophores
augmentation
wavelengths
nonuniformity
Light sources
light sources
plane waves

ASJC Scopus subject areas

  • Bioengineering
  • Electrical and Electronic Engineering
  • Materials Chemistry
  • Condensed Matter Physics

Cite this

Chaudhery, V., Lu, M., Pokhriyal, A., Schulz, S. C., & Cunningham, B. T. (2011). Optimization of photonic crystal enhanced fluorescence by excitation laser angle scanning. In 2011 11th IEEE International Conference on Nanotechnology, NANO 2011 (pp. 282-285). [6144349] (Proceedings of the IEEE Conference on Nanotechnology). https://doi.org/10.1109/NANO.2011.6144349

Optimization of photonic crystal enhanced fluorescence by excitation laser angle scanning. / Chaudhery, Vikram; Lu, Meng; Pokhriyal, Anusha; Schulz, Stephen C.; Cunningham, Brian T.

2011 11th IEEE International Conference on Nanotechnology, NANO 2011. 2011. p. 282-285 6144349 (Proceedings of the IEEE Conference on Nanotechnology).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chaudhery, V, Lu, M, Pokhriyal, A, Schulz, SC & Cunningham, BT 2011, Optimization of photonic crystal enhanced fluorescence by excitation laser angle scanning. in 2011 11th IEEE International Conference on Nanotechnology, NANO 2011., 6144349, Proceedings of the IEEE Conference on Nanotechnology, pp. 282-285, 2011 11th IEEE International Conference on Nanotechnology, NANO 2011, Portland, OR, United States, 8/15/11. https://doi.org/10.1109/NANO.2011.6144349
Chaudhery V, Lu M, Pokhriyal A, Schulz SC, Cunningham BT. Optimization of photonic crystal enhanced fluorescence by excitation laser angle scanning. In 2011 11th IEEE International Conference on Nanotechnology, NANO 2011. 2011. p. 282-285. 6144349. (Proceedings of the IEEE Conference on Nanotechnology). https://doi.org/10.1109/NANO.2011.6144349
Chaudhery, Vikram ; Lu, Meng ; Pokhriyal, Anusha ; Schulz, Stephen C. ; Cunningham, Brian T. / Optimization of photonic crystal enhanced fluorescence by excitation laser angle scanning. 2011 11th IEEE International Conference on Nanotechnology, NANO 2011. 2011. pp. 282-285 (Proceedings of the IEEE Conference on Nanotechnology).
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