Optimization of butanol production from tropical maize stalk juice by fermentation with Clostridium beijerinckii NCIMB 8052

Research output: Contribution to journalArticle

Abstract

Mixed sugars from tropical maize stalk juice were used to carry out butanol fermentation with Clostridium beijerinckii NCIMB 8052. Batch experiments employing central composite design (CCD) and response surface methodology (RSM) optimization were performed to evaluate effects of three factors, i.e. pH, initial total sugar concentration, and agitation rate on butanol production. Optimum conditions of pH 6.7, sugar concentration 42.2. g/L and agitation rate 48. rpm were predicted, under which a maximum butanol yield of 0.27. g/g-sugar was estimated. Further experiments demonstrated that higher agitation facilitated acetone production, leading to lower butanol selectivity in total acetone-butanol-ethanol (ABE). While glucose and fructose are more preferable by C. beijerinckii, sucrose can also be easily degraded by the microorganism. This study indicated that RSM is a useful approach for optimizing operational conditions for butanol production, and demonstrated that tropical maize, with high yield of biomass and stalk sugars, is a promising biofuel crop.

Original languageEnglish (US)
Pages (from-to)9985-9990
Number of pages6
JournalBioresource Technology
Volume102
Issue number21
DOIs
StatePublished - Nov 1 2011

Keywords

  • Butanol production
  • Clostridium beijerinckii
  • Optimization
  • Response surface methodology (RSM)
  • Tropical maize stalk juice

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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