Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology

Luz Maria Paucar-Menacho; Mark A. Berhow; José Marcos Gontijo Mandarino; Elvira Gonzalez de Mejia; Yoon Kil Chang

doi:10.1016/j.foodchem.2009.07.011

Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology

Luz Maria Paucar-Menacho, Mark A. Berhow, José Marcos Gontijo Mandarino, Elvira Gonzalez de Mejia, Yoon Kil Chang

Research output: Contribution to journal › Article › peer-review

Abstract

The objective was to optimise the effect of germination time and temperature on the concentration of soluble protein, lunasin, BBI, lectin, saponins and isoflavones in soybean seeds from cultivar BRS 133. Isoflavone and saponin concentrations were analysed by HPLC. Lunasin, Bowman-Birk inhibitor and lectin were analysed by ELISA and western blot. The effects of the variations in germination time and temperature on bioactive compounds were analysed using the response surface methodology (RSM), with a 2² central composite rotational design. Germination of soybean for 42 h at 25 °C resulted in an increase of 61.7% of lunasin, decrease of 58.7% in lectin and 70.0% in lipoxygenase activity. Optimal increases in the concentrations of isoflavone aglycones were observed in combination of 63 h of germination and 30 °C. A significant increase of 32.2% in the concentration of soy saponins was observed in combination of 42 h of germination at 25 °C.

Original language	English (US)
Pages (from-to)	636-642
Number of pages	7
Journal	Food chemistry
Volume	119
Issue number	2
DOIs	https://doi.org/10.1016/j.foodchem.2009.07.011
State	Published - Mar 15 2010

Keywords

Bowman-Birk inhibitor
Germination
Isoflavone
Lectin
Lunasin
Saponin
Soy peptides
Soybean

ASJC Scopus subject areas

Analytical Chemistry
Food Science

Online availability

10.1016/j.foodchem.2009.07.011

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Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology. / Paucar-Menacho, Luz Maria; Berhow, Mark A.; Mandarino, José Marcos Gontijo et al.

In: Food chemistry, Vol. 119, No. 2, 15.03.2010, p. 636-642.

Research output: Contribution to journal › Article › peer-review

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title = "Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology",

abstract = "The objective was to optimise the effect of germination time and temperature on the concentration of soluble protein, lunasin, BBI, lectin, saponins and isoflavones in soybean seeds from cultivar BRS 133. Isoflavone and saponin concentrations were analysed by HPLC. Lunasin, Bowman-Birk inhibitor and lectin were analysed by ELISA and western blot. The effects of the variations in germination time and temperature on bioactive compounds were analysed using the response surface methodology (RSM), with a 22 central composite rotational design. Germination of soybean for 42 h at 25 °C resulted in an increase of 61.7% of lunasin, decrease of 58.7% in lectin and 70.0% in lipoxygenase activity. Optimal increases in the concentrations of isoflavone aglycones were observed in combination of 63 h of germination and 30 °C. A significant increase of 32.2% in the concentration of soy saponins was observed in combination of 42 h of germination at 25 °C.",

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note = "Funding Information: The authors wish to thank CAPES-PEC PG for Granting Luz Maria Paucar-Menacho{\textquoteright}s scholarship and to thanks Rodolfo Rohr Neto (SoSoja do Brasil Ltda.) and Kenji S. Narumiya (Sun Foods-Brazil) for the financial support. Embrapa Soybean – The National Center for Soybean Research, Brazil. Embrapa Technology Transfer, Brazil, for the donation of soybean BRS 133. Unicamp{\textquoteright}s Foundation for Teaching, Research and Extension (FAEPEX) Grant No. 149/08. ",

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AU - Mandarino, José Marcos Gontijo

AU - de Mejia, Elvira Gonzalez

AU - Chang, Yoon Kil

N1 - Funding Information: The authors wish to thank CAPES-PEC PG for Granting Luz Maria Paucar-Menacho’s scholarship and to thanks Rodolfo Rohr Neto (SoSoja do Brasil Ltda.) and Kenji S. Narumiya (Sun Foods-Brazil) for the financial support. Embrapa Soybean – The National Center for Soybean Research, Brazil. Embrapa Technology Transfer, Brazil, for the donation of soybean BRS 133. Unicamp’s Foundation for Teaching, Research and Extension (FAEPEX) Grant No. 149/08.

PY - 2010/3/15

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N2 - The objective was to optimise the effect of germination time and temperature on the concentration of soluble protein, lunasin, BBI, lectin, saponins and isoflavones in soybean seeds from cultivar BRS 133. Isoflavone and saponin concentrations were analysed by HPLC. Lunasin, Bowman-Birk inhibitor and lectin were analysed by ELISA and western blot. The effects of the variations in germination time and temperature on bioactive compounds were analysed using the response surface methodology (RSM), with a 22 central composite rotational design. Germination of soybean for 42 h at 25 °C resulted in an increase of 61.7% of lunasin, decrease of 58.7% in lectin and 70.0% in lipoxygenase activity. Optimal increases in the concentrations of isoflavone aglycones were observed in combination of 63 h of germination and 30 °C. A significant increase of 32.2% in the concentration of soy saponins was observed in combination of 42 h of germination at 25 °C.

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Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology

Abstract

Keywords

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