Abstract
We have developed a novel optical coding technology for massively parallel and high-throughput analysis of biological molecules. Its unprecedented multiplexing capability is based on the unique optical properties of semiconductor quantum dots (QDs) and the ability to incorporate multicolor QDs into small polymer beads at precisely controlled ratios. The use of 10 intensity levels and 6 colors could theoretically code one million nucleic acid or protein sequences. Imaging and spectroscopic studies indicate that the QD tagged beads are highly uniform and reproducible, yielding bead identification accuracies as high as 99.99% under favorable conditions. DNA hybridization results demonstrate that the coding and target signals can be simultaneously read at the single-bead level. This spectral coding technology is expected to open new opportunities in gene expression studies, high-throughput screening, and medical diagnosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 210-217 |
| Number of pages | 8 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 4626 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
| Event | Biomedical Nanotechnology Architectures and Applications - San Jose, CA, United States Duration: Jan 20 2002 → Jan 24 2002 |
Keywords
- Biomedical diagnostics
- Drug screening
- Genomics
- High-throughput
- Multiplexing
- Nanocrystals
- Proteomics
- Quantum dots
- Spectral barcoding
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering