Optical microscopy and spectroscopy for epigenetic modifications in single living cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Optical imaging with high spatiotemporal resolution and analytical accuracy is becoming the mainstay of tools capable of deciphering molecular dynamics and activities in single living cells. Over the past decades, information obtained by optical imaging has greatly enriched and reshaped our knowledge of biology and medicine. Investigating epigenetic modifications by optical microscopy and spectroscopy is expected to be the wave of the future or might even become the norm to complement biomedical practice. Independent of classical genetic mechanisms, epigenetics has recently drawn substantial attention due to its extensive involvement in physiological and pathological processes, as well as its reversibility. In order to understand the real-time behaviors of epigenetic regulation, nanoscale inspection at the sub-second timescale is imperative. In this chapter we discuss the basics of state-of-the-art optical methods for life science research and their potential applications in imaging live-cell epigenetics. Moreover, with established experience in single-molecule detection, we provide practical guidance on how to choose and adapt optical instrumenta-tions for different applications. Last, recent advancements and representative examples in sensing live-cell epigenetics are reviewed.

Original languageEnglish (US)
Title of host publicationMethods in Pharmacology and Toxicology
PublisherHumana Press Inc.
Pages135-154
Number of pages20
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Publication series

NameMethods in Pharmacology and Toxicology
ISSN (Print)1557-2153
ISSN (Electronic)1940-6053

Keywords

  • Chromatin dynamics
  • DNA methylation
  • FCS
  • FRAP
  • FRET
  • Histone modification
  • Light-sheet microscopy
  • Live-cell imaging
  • Phototoxicity
  • Single-molecule detection
  • Spatiotemporal resolution
  • Super-resolution microscopy

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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  • Cite this

    Cui, Y., & Irudayaraj, J. (2017). Optical microscopy and spectroscopy for epigenetic modifications in single living cells. In Methods in Pharmacology and Toxicology (pp. 135-154). (Methods in Pharmacology and Toxicology). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6743-8_9