Optical methods for measuring single-molecule orientation and position: Implications for super-resolution microscopy

Matthew D. Lew, Mikael P. Backlund, Adam S. Backer, W. E. Moerner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The quadrated pupil and double-helix PSF are modifications of epifluorescence microscopes that enable precise measurements of the single-molecule orientation. We explore how position estimation beyond the diffraction limit is affected by single-molecule orientation within cells.

Original languageEnglish (US)
Title of host publicationFrontiers in Optics, FIO 2013
PublisherOptical Society of America
ISBN (Print)9781557529879
StatePublished - 2013
Externally publishedYes
EventFrontiers in Optics, FIO 2013 - Orlando, FL, United States
Duration: Oct 6 2013Oct 10 2013

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Other

OtherFrontiers in Optics, FIO 2013
Country/TerritoryUnited States
CityOrlando, FL
Period10/6/1310/10/13

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'Optical methods for measuring single-molecule orientation and position: Implications for super-resolution microscopy'. Together they form a unique fingerprint.

Cite this