Optical hyperspectral imaging in microscopy and spectroscopy – A review of data acquisition

Liang Gao, R. Theodore Smith

Research output: Contribution to journalReview articlepeer-review


Rather than simply acting as a photographic camera capturing two-dimensional (x, y) intensity images or a spectrometer acquiring spectra (λ), a hyperspectral imager measures entire three-dimensional (x, y, λ) datacubes for multivariate analysis, providing structural, molecular, and functional information about biological cells or tissue with unprecedented detail. Such data also gives clinical insights for disease diagnosis and treatment. We summarize the principles underpinning this technology, highlight its practical implementation, and discuss its recent applications at microscopic to macroscopic scales. (Image presented) Datacube acquisition strategies in hyperspectral imaging x, y, spatial coordinates; λ, wavelength.

Original languageEnglish (US)
Pages (from-to)441-456
Number of pages16
JournalJournal of Biophotonics
Issue number6
StatePublished - Jun 1 2015
Externally publishedYes


  • Biomedical imaging
  • Hyperspectral imaging
  • Multispectral imaging

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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