Optical detection of thymine dinucleoside monophosphate and its cis-syn photodimer by inorganic nanoparticles

K. K. Caswell, Rahina Mahtab, Catherine J. Murphy

Research output: Contribution to journalArticlepeer-review

Abstract

The Watson-Crick DNA double helix is an averaged ideal of multitudinous natural sequence-directed local structural deviations. By effectively derailing normal cellular physiological processes, damaged bases can induce noncanonical irregularities in the local structure of DNA if not efficiently repaired. Pyrimidine bases, especially thymine, are prone to dimerization when exposed to ultraviolet light. A [2 + 2] photocyclo-addition between adjacent thymine bases predominantly produces the cis-syn photodimer. These lesions, implicated in skin cancer, bend DNA by ∼30° due to their structural and conformational changes. Such changes in molecular properties can be detected by differential quenching of CdS nanoparticle luminescence and by surface-enhanced Raman scattering spectroscopy on metal nanoparticle substrates.

Original languageEnglish (US)
Pages (from-to)407-415
Number of pages9
JournalJournal of Fluorescence
Volume14
Issue number4
DOIs
StatePublished - Jul 2004
Externally publishedYes

Keywords

  • CdS
  • Luminescence
  • Photodimer
  • SERS
  • Thymine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

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