TY - JOUR
T1 - Optical biopsy in human pancreatobiliary tissue using optical coherence tomography
AU - Tearney, G. J.
AU - Brezinski, M. E.
AU - Southern, J. F.
AU - Bouma, B. E.
AU - Boppart, S. A.
AU - Fujimoto, J. G.
N1 - Funding Information:
Manuscript received July 25, 1997; revised manuscript received January 15, 1998; accepte d February 23, 1998. From the Harvad Mredical School and Cardiac Unit, Massachusetts General Hospital, Boston, Massachusetts 02114; and Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge , Massachuse tts 02139. This rese arch is supported in part by grants from the National Institutes of Health, contracts NIH-9-R O 1-E Y11289-11 (to J.G.F.), NH-1-RI01-CA75289-01 (to J.G.F. and M.E.B.), NIH-1-R29-HL55 686-01A 1 (to M.E.B.), NIH-RO 1-AR44812-01 (to M.E.B.),the Of® ce ofNal Raeseavrc,hMedical Free Electron Lase r Program, grant N000014-97-1 -1066, and the Whittaker Foundation contract 96-0205 (to M.E.B.). Address for reprint requests: James G. Fujimoto, Massachusetts Institute of Technolog, Byuilding 36-357, 77 Massachusetts Ave - nue, Cambridge , Massachuse tts 02139.
PY - 1998
Y1 - 1998
N2 - Optical coherence tomography (OCT) is a new technique for performing high-resolution, cross-sectional tomographic imaging in human tissue. OCT is analogous to ultrasound B mode imaging except that it uses light rather than acoustical waves. As a result, OCT has over 10 times the resolution of currently available clinical high-resolution cross-sectional imaging technologies. In this work, we investigate the capability of OCT to differentiate the architectural morphology of pancreatobiliary tissues. Normal pancreatobiliary tissues, including the gallbladder, common bile duct, pancreatic duct, and pancreas were taken postmortem and imaged using OCT. Images were compared to corresponding histology to confirm tissue identity. Microstructure was delineated in different tissues, including tissue layers, glands, submucosal microvasculature, and pancreatic islets of Langerhans. The ability of OCT to provide high-resolution imaging of pancreatobiliary architectural morphology suggests the feasibility of using OCT as a powerful diagnostic endoscopic imaging technology to image early stages of pancreatobiliary disease.
AB - Optical coherence tomography (OCT) is a new technique for performing high-resolution, cross-sectional tomographic imaging in human tissue. OCT is analogous to ultrasound B mode imaging except that it uses light rather than acoustical waves. As a result, OCT has over 10 times the resolution of currently available clinical high-resolution cross-sectional imaging technologies. In this work, we investigate the capability of OCT to differentiate the architectural morphology of pancreatobiliary tissues. Normal pancreatobiliary tissues, including the gallbladder, common bile duct, pancreatic duct, and pancreas were taken postmortem and imaged using OCT. Images were compared to corresponding histology to confirm tissue identity. Microstructure was delineated in different tissues, including tissue layers, glands, submucosal microvasculature, and pancreatic islets of Langerhans. The ability of OCT to provide high-resolution imaging of pancreatobiliary architectural morphology suggests the feasibility of using OCT as a powerful diagnostic endoscopic imaging technology to image early stages of pancreatobiliary disease.
KW - Imaging
KW - Microscopy
KW - Optical biopsy
KW - Optical coherence tomography
KW - Pancreatobiliary tissue
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U2 - 10.1023/A:1018891304453
DO - 10.1023/A:1018891304453
M3 - Article
C2 - 9635607
AN - SCOPUS:0031799826
VL - 43
SP - 1193
EP - 1199
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
SN - 0163-2116
IS - 6
ER -