Opportunities for bead-based multiplex assays in veterinary diagnostic laboratories

Jane Christopher-Hennings, Karla P.C. Araujo, Carlos J.H. Souza, Ying Fang, Steven Lawson, Eric A. Nelson, Travis Clement, Michael Dunn, Joan K. Lunney

Research output: Contribution to journalArticle

Abstract

Bead-based multiplex assays (BBMAs) are applicable for high throughput, simultaneous detection of multiple analytes in solution (from several to 50-500 analytes within a single, small sample volume). Currently, few assays are commercially available for veterinary applications, but they are available to identify and measure various cytokines, growth factors and their receptors, inflammatory proteins, kinases and inhibitors, neurobiology proteins, and pathogens and antibodies in human beings, nonhuman primates, and rodent species. In veterinary medicine, various nucleic acid and protein-coupled beads can be used in, or for the development of, antigen and antibody BBMAs, with the advantage that more data can be collected using approximately the same amount of labor as used for other antigen and antibody assays. Veterinary-related BBMAs could be used for detection of pathogens, genotyping, measurement of hormone levels, and in disease surveillance and vaccine assessment. It will be important to evaluate whether BBMAs are "fit for purpose," how costs and efficiencies compare between assays, which assays are published or commercially available for specific veterinary applications, and what procedures are involved in the development of the assays. It is expected that many veterinary-related BBMAs will be published and/or become commercially available in the next few years. The current review summarizes the BBMA technology and some of the currently available BBMAs developed for veterinary settings. Some of the human diagnostic BBMAs are also described, providing an example of possible templates for future development of new veterinary-related BBMAs.

Original languageEnglish (US)
Pages (from-to)671-691
Number of pages21
JournalJournal of Veterinary Diagnostic Investigation
Volume25
Issue number6
DOIs
StatePublished - Nov 1 2013
Externally publishedYes

Keywords

  • Bead assay
  • Luminex
  • cytometric bead array
  • diagnostics
  • multiplex
  • polymerase chain reaction
  • xMAP

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Opportunities for bead-based multiplex assays in veterinary diagnostic laboratories'. Together they form a unique fingerprint.

  • Cite this

    Christopher-Hennings, J., Araujo, K. P. C., Souza, C. J. H., Fang, Y., Lawson, S., Nelson, E. A., Clement, T., Dunn, M., & Lunney, J. K. (2013). Opportunities for bead-based multiplex assays in veterinary diagnostic laboratories. Journal of Veterinary Diagnostic Investigation, 25(6), 671-691. https://doi.org/10.1177/1040638713507256