One-stop genomic DNA extraction by salicylic acid-coated magnetic nanoparticles

Zhongwu Zhou, Ulhas S. Kadam, Joseph Irudayaraj

Research output: Contribution to journalArticlepeer-review

Abstract

Salicylic acid-coated magnetic nanoparticles were prepared via a modified one-step synthesis and used for a one-stop extraction of genomic DNA from mammalian cells. The synthesized magnetic particles were used for magnetic separation of cells from the media by nonspecific binding of the particles as well as extraction of genomic DNA from the lysate. The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reaction. The entire process of extraction and isolation can be completed within 30 min. Compared with traditional methods based on centrifugation and filtration, the established method is fast, simple, reliable, and environmentally friendly.

Original languageEnglish (US)
Pages (from-to)249-252
Number of pages4
JournalAnalytical Biochemistry
Volume442
Issue number2
DOIs
StatePublished - 2013
Externally publishedYes

Keywords

  • Extraction
  • Genomic DNA
  • Magnetic nanoparticles
  • Salicylic acid
  • Simple and rapid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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