Abstract
Lantibiotic synthetases generate intramolecular thioether cross-links within peptides through the Michael-type addition of cysteines onto dehydroamino acids originating from Ser and Thr. Presented here is an assay that readily distinguishes between enzymatic and nonenzymatic formation of these cross-links. The results demonstrate unequivocally that lacticin 481 synthetase can generate non-native thioether cross-links.
Original language | English (US) |
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Pages (from-to) | 3343-3346 |
Number of pages | 4 |
Journal | Organic Letters |
Volume | 9 |
Issue number | 17 |
DOIs | |
State | Published - Aug 16 2007 |
ASJC Scopus subject areas
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry