Lantibiotic synthetases generate intramolecular thioether cross-links within peptides through the Michael-type addition of cysteines onto dehydroamino acids originating from Ser and Thr. Presented here is an assay that readily distinguishes between enzymatic and nonenzymatic formation of these cross-links. The results demonstrate unequivocally that lacticin 481 synthetase can generate non-native thioether cross-links.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Aug 16 2007|
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry