On the interaction of [Ru(phen)2dppz]2+ (dppz = dipyrido[3,2-a:2',3' c]phenazine) with different oligonucleotides

Rajesh B. Nair, Catherine J. Murphy

Research output: Contribution to journalArticlepeer-review

Abstract

Luminescent titrations have been performed on a 'molecular light switch for DNA', [Ru(phen)2dppz]2+ (dppz= dipyrido[3,2-a:2',3'-c]phenazine), with several different 16-bp oligonucleotides having sequence-directed shape ('straight', 'bent' and 'kinked'). Binding constants and binding site sizes are consistent with metal complex intercalation for all oligonucleotides. The emission maxima and relative intensities upon saturation are consistent with trends found for long DNAs based on relative GC content. [Ru(phen)2dppz]2+ has a significantly higher binding constant and larger site size for the 'kinked' oligonucleotide than for the 'straight' and 'bent', suggesting that local DNA deformation plays a role in the interaction of metallointercalators with DNA.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalJournal of Inorganic Biochemistry
Volume69
Issue number1-2
DOIs
StatePublished - Feb 1 1998
Externally publishedYes

Keywords

  • DNA probe
  • Dppz
  • Oligonucleotides
  • Ru

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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