On the importance of image formation optics in the design of infrared spectroscopic imaging systems

David Mayerich, Thomas Van Dijk, Michael J. Walsh, Matthew V. Schulmerich, P. Scott Carney, Rohit Bhargava

Research output: Contribution to journalArticlepeer-review


Infrared spectroscopic imaging provides micron-scale spatial resolution with molecular contrast. While recent work demonstrates that sample morphology affects the recorded spectrum, considerably less attention has been focused on the effects of the optics, including the condenser and objective. This analysis is extremely important, since it will be possible to understand effects on recorded data and provides insight for reducing optical effects through rigorous microscope design. Here, we present a theoretical description and experimental results that demonstrate the effects of commonly-employed cassegranian optics on recorded spectra. We first combine an explicit model of image formation and a method for quantifying and visualizing the deviations in recorded spectra as a function of microscope optics. We then verify these simulations with measurements obtained from spatially heterogeneous samples. The deviation of the computed spectrum from the ideal case is quantified via a map which we call a deviation map. The deviation map is obtained as a function of optical elements by systematic simulations. Examination of deviation maps demonstrates that the optimal optical configuration for minimal deviation is contrary to prevailing practice in which throughput is maximized for an instrument without a sample. This report should be helpful for understanding recorded spectra as a function of the optics, the analytical limits of recorded data determined by the optical design, and potential routes for optimization of imaging systems. This journal is

Original languageEnglish (US)
Pages (from-to)4031-4036
Number of pages6
Issue number16
StatePublished - Jul 14 2014

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry


Dive into the research topics of 'On the importance of image formation optics in the design of infrared spectroscopic imaging systems'. Together they form a unique fingerprint.

Cite this