On chip analysis of CNS lymphoma in cerebrospinal fluid

Anna Turetsky, Kyungheon Lee, Jun Song, Randy J. Giedt, Eunha Kim, Alexandra E. Kovach, Ephraim P. Hochberg, Cesar M. Castro, Hakho Lee, Ralph Weissleder

Research output: Contribution to journalArticle

Abstract

Molecular profiling of central nervous system lymphomas in cerebrospinal fluid (CSF) samples can be challenging due to the paucicellular and limited nature of the samples. Presented herein is a microfluidic platform for complete CSF lymphoid cell analysis, including single cell capture in sub-nanoliter traps, and molecular and chemotherapeutic response profiling via on-chip imaging, all in less than one hour. The system can detect scant lymphoma cells and quantitate their kappa/lambda immunoglobulin light chain restriction patterns. The approach can be further customized for measurement of additional biomarkers, such as those for differential diagnosis of lymphoma subtypes or for prognosis, as well as for imaging exposure to experimental drugs.

Original languageEnglish (US)
Pages (from-to)796-804
Number of pages9
JournalTheranostics
Volume5
Issue number8
DOIs
StatePublished - 2015

Keywords

  • Cerebrospinal fluid
  • Drug testing
  • Lymphoma
  • Microfluidics
  • Point-of-care

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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  • Cite this

    Turetsky, A., Lee, K., Song, J., Giedt, R. J., Kim, E., Kovach, A. E., Hochberg, E. P., Castro, C. M., Lee, H., & Weissleder, R. (2015). On chip analysis of CNS lymphoma in cerebrospinal fluid. Theranostics, 5(8), 796-804. https://doi.org/10.7150/thno.11220