o-Diphenol: Oxygen oxidoreductase from leaves of sugar cane

James Coombs, Carl Baldry, Christopher Bucke, Stephen P. Long

Research output: Contribution to journalArticle

Abstract

A non-particulate o-diphenol: O2 oxidoreductase (phenolase) has been isolated from leaves of sugar cane. Gel filtration produced two fractions MW 32000 and 130000. The preferred substrate was chlorogenic acid. Other o-diphenols (caffeic acid, catechol, pyrogallol, dihydroxyphenylalanine) all of which were slowly oxidized when tested alone, increased the rates of O2 consumption obtained with catalytic amounts of chlorogenic acid. Both enzyme fractions were inhibited by thiols; thioglycollate, which acted in a non-competitive manner, was most effective.

Original languageEnglish (US)
Pages (from-to)2703-2708
Number of pages6
JournalPhytochemistry
Volume13
Issue number12
DOIs
StatePublished - Dec 1974
Externally publishedYes

Fingerprint

Sugar cane
Chlorogenic Acid
Canes
oxidoreductases
chlorogenic acid
sugarcane
Oxidoreductases
Pyrogallol
Oxygen
dihydroxyphenylalanine
Thioglycolates
oxygen
pyrogallol
Dihydroxyphenylalanine
catechol
caffeic acid
thiols
Sulfhydryl Compounds
Gel Chromatography
leaves

Keywords

  • Gramineae
  • MW determination, chlorogenic acid, substrate specificity, inhibition, thioglycollic acid.
  • Saccharum sp.
  • phenoloxidase
  • sugar cane

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

Cite this

o-Diphenol : Oxygen oxidoreductase from leaves of sugar cane. / Coombs, James; Baldry, Carl; Bucke, Christopher; Long, Stephen P.

In: Phytochemistry, Vol. 13, No. 12, 12.1974, p. 2703-2708.

Research output: Contribution to journalArticle

Coombs, James ; Baldry, Carl ; Bucke, Christopher ; Long, Stephen P. / o-Diphenol : Oxygen oxidoreductase from leaves of sugar cane. In: Phytochemistry. 1974 ; Vol. 13, No. 12. pp. 2703-2708.
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