Nucleotide sequence of the first cosmid from the Mycobacterium leprae genome project: structure and function of the Rif‐Str regions

N. Honoré, S. Bergh, S. Chanteau, F. Doucet‐Populaire, K. Eiglmeir, T. Garnier, C. Georges, P. Launois, T. Limpaiboon, S. Newton, K. Niang, P. del Portillo, G. R. Ramesh, P. Reddi, P. R. Ridel, N. Sittisombut, S. Wu‐Hunte, S. T. Cole

Research output: Contribution to journalArticlepeer-review

Abstract

The nucleotide sequence of cosmid B1790, carrying the Rif‐Str regions of the Mycobacterium leprae chromosome, has been determined. Twelve open reading frames were identified in the 36716bp sequence, representing 40% of the coding capacity. Five ribosomal proteins, two elongation factors and the β and β'subunits of RNA polymerase have been characterized and two novel genes were found. One of these encodes a member of the so‐called ABC family of ATP‐binding proteins while the other appears to encode an enzyme involved in repairing genomic lesions caused by free radicals. This finding may well be significant as M. leprae, an intracellular pathogen, lives within macrophages.

Original languageEnglish (US)
Pages (from-to)207-214
Number of pages8
JournalMolecular Microbiology
Volume7
Issue number2
DOIs
StatePublished - Jan 1993
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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