Nucleosomes undergo slow spontaneous gaping

Thuy T.M. Ngo, Taekjip Ha

Research output: Contribution to journalArticlepeer-review


In eukaryotes, DNA is packaged into a basic unit, the nucleosome which consists of 147 bp of DNA wrapped around a histone octamer composed of two copies each of the histones H2A, H2B, H3 and H4. Nucleosome structures are diverse not only by histone variants, histone modifications, histone composition but also through accommodating different conformational states such as DNA breathing and dimer splitting. Variation in nucleosome structures allows it to perform a variety of cellular functions. Here, we identified a novel spontaneous conformational switching of nucleosomes under physiological conditions using single-molecule FRET. Using FRET probes placed at various positions on the nucleosomal DNA to monitor conformation of the nucleosome over a long period of time (30-60 min) at various ionic conditions, we identified conformational changes we refer to as nucleosome gaping. Gaping transitions are distinct from nucleosome breathing, sliding or tightening. Gaping modes switch along the direction normal to the DNA plane through about 5-10 angstroms and at minutes (1-10 min) time scale. This conformational transition, which has not been observed previously, may be potentially important for enzymatic reactions/transactions on nucleosomal substrate and the formation of multiple compression forms of chromatin fibers.

Original languageEnglish (US)
Pages (from-to)3964-3971
Number of pages8
JournalNucleic acids research
Issue number8
StatePublished - Mar 19 2015

ASJC Scopus subject areas

  • Genetics


Dive into the research topics of 'Nucleosomes undergo slow spontaneous gaping'. Together they form a unique fingerprint.

Cite this