Nucleic acid scanning-by-hybridization of enterohemorrhagic Escherichia coll isolates using oligodeoxynucleotide arrays

N. M. Salazar, G. Caetano-Anollés

Research output: Contribution to journalArticle

Abstract

Nucleic acid scanning by hybridization (NASBH) is a non-electrophoretic typing strategy that uses gridded oligonucleotides to reproducibly characterize arbitrarily amplified nucleic acid sequences. Membrane-bound arrays of terminally-degenerate oligonucleotides were hybridized to DNA amplification fingerprinting (DAF) products from enterohemorrhagic Escherichia coli O157:H7 isolates. Numerical and cluster analysis of 64 isolates, selected by DAF to represent a single dominant amplification type identified 14 hybridization types. Results show that NASBH is a powerful alternative for the identification of closely related bacteria, can be used successfully in epidemiological studies, and holds potential in general nucleic acid diagnostics.

Original languageEnglish (US)
Pages (from-to)5056-5057
Number of pages2
JournalNucleic acids research
Volume24
Issue number24
StatePublished - Dec 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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