Abstract
Nucleic acid scanning by hybridization (NASBH) is a non-electrophoretic typing strategy that uses gridded oligonucleotides to reproducibly characterize arbitrarily amplified nucleic acid sequences. Membrane-bound arrays of terminally-degenerate oligonucleotides were hybridized to DNA amplification fingerprinting (DAF) products from enterohemorrhagic Escherichia coli O157:H7 isolates. Numerical and cluster analysis of 64 isolates, selected by DAF to represent a single dominant amplification type identified 14 hybridization types. Results show that NASBH is a powerful alternative for the identification of closely related bacteria, can be used successfully in epidemiological studies, and holds potential in general nucleic acid diagnostics.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5056-5057 |
| Number of pages | 2 |
| Journal | Nucleic acids research |
| Volume | 24 |
| Issue number | 24 |
| DOIs | |
| State | Published - Dec 15 1996 |
| Externally published | Yes |
ASJC Scopus subject areas
- Genetics