Nuclear Migration of NF-kappa B Correlates with TNF-alpha mRNA Accumulation

H Albrecht, L B Schook, C V Jongeneel

Research output: Contribution to journalArticlepeer-review


Previous work on the transcriptional regulation of the mouse TNF-alpha promoter had indicated a major role for the NF-kappa B transcription factor in the induction of the gene by endotoxin. However, similar studies using the human promoter failed to establish a role for this factor. We measured the nuclear migration of NF-kappa B and the accumulation of TNF-alpha mRNA in murine T lymphoblasts and bone marrow-derived macrophages (BMDM) under different activation conditions, seeking to establish a correlation. Activation of NF-kappa B and accumulation of TNF-alpha mRNA correlated semiquantitatively under the following conditions: (1) inhibition of NF-kappa B by dithiocarbamates; (2) induction of TNF synthesis by taxol; (3) partial induction of TNF-alpha mRNA by various inducers in macrophages from lpsd mice; and (4) inhibition of NF-kappa B activation by a protease inhibitor. However, inhibition of TNF-alpha mRNA accumulation by cAMP inducers had no effect on NF-kappa B induction. We conclude that NF-kappa B translocation is necessary, but not sufficient for the transcriptional induction of the TNF-alpha gene by LPS in macrophages or by phorbol ester and ionomycin in T lymphocytes.

Original languageEnglish (US)
Pages (from-to)64-71
Number of pages8
JournalJournal of inflammation
Issue number1
StatePublished - 1995


  • Animals
  • Base Sequence
  • Biological Transport
  • Bone Marrow Cells
  • Cell Nucleus/metabolism
  • Cells, Cultured
  • Cyclic AMP/metabolism
  • Free Radical Scavengers/pharmacology
  • Macrophages/metabolism
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • NF-kappa B/metabolism
  • Nocodazole/pharmacology
  • Paclitaxel/pharmacology
  • RNA, Messenger/metabolism
  • Serine Proteinase Inhibitors/pharmacology
  • T-Lymphocytes/metabolism
  • Tosylphenylalanyl Chloromethyl Ketone/pharmacology
  • Tumor Necrosis Factor-alpha/genetics


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