Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition

Zhi Chen; Shaoning Liu; Wenbo Sun; Lei Chen; Dongwan Yoo; Feng Li; Sufang Ren; Lihui Guo; Xiaoyan Cong; Jun Li; Shun Zhou; Jiaqiang Wu; Yijun Du; Jinbao Wang

doi:10.1016/j.virol.2016.07.008

Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition

Zhi Chen, Shaoning Liu, Wenbo Sun, Lei Chen, Dongwan Yoo, Feng Li, Sufang Ren, Lihui Guo, Xiaoyan Cong, Jun Li, Shun Zhou, Jiaqiang Wu, Yijun Du, Jinbao Wang

Research output: Contribution to journal › Article › peer-review

Abstract

The nonstructural protein 1α (NSP1α) of porcine reproductive and respiratory syndrome virus (PRRSV) is a nucleo-cytoplasmic protein that suppresses the production of type I interferon (IFN). In this study, we investigated the relationship between the subcellular distribution of NSP1α and its inhibition of type I IFN. NSP1α was found to contain the classical nuclear export signal (NES) and NSP1α nuclear export was CRM-1-mediated. NSP1α was shuttling between the nucleus and cytoplasm. We also showed that the nuclear export of NSP1α was necessary for its ability for type I IFN inhibition. NSP1α was also found to interact with CBP, which implies a possible mechanism of CBP degradation by NSP1α. Taken together, our results describe a novel mechanism of PRRSV NSP1α for type I IFN inhibition and suppression of the host innate antiviral response.

Original language	English (US)
Pages (from-to)	278-287
Number of pages	10
Journal	Virology
Volume	499
DOIs	https://doi.org/10.1016/j.virol.2016.07.008
State	Published - Dec 1 2016

Keywords

NSP1α
Nuclear export signal (NES)
PRRSV
Type I IFN

ASJC Scopus subject areas

Virology

Online availability

10.1016/j.virol.2016.07.008

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@article{9c1476a2a0c34fdf94a34c1355877d17,

title = "Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition",

abstract = "The nonstructural protein 1α (NSP1α) of porcine reproductive and respiratory syndrome virus (PRRSV) is a nucleo-cytoplasmic protein that suppresses the production of type I interferon (IFN). In this study, we investigated the relationship between the subcellular distribution of NSP1α and its inhibition of type I IFN. NSP1α was found to contain the classical nuclear export signal (NES) and NSP1α nuclear export was CRM-1-mediated. NSP1α was shuttling between the nucleus and cytoplasm. We also showed that the nuclear export of NSP1α was necessary for its ability for type I IFN inhibition. NSP1α was also found to interact with CBP, which implies a possible mechanism of CBP degradation by NSP1α. Taken together, our results describe a novel mechanism of PRRSV NSP1α for type I IFN inhibition and suppression of the host innate antiviral response.",

keywords = "NSP1α, Nuclear export signal (NES), PRRSV, Type I IFN",

author = "Zhi Chen and Shaoning Liu and Wenbo Sun and Lei Chen and Dongwan Yoo and Feng Li and Sufang Ren and Lihui Guo and Xiaoyan Cong and Jun Li and Shun Zhou and Jiaqiang Wu and Yijun Du and Jinbao Wang",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China ( 31170146 , 31602076 , 31370189 and 31572550 ), Research Award Fund for Outstanding Young Scientist of Shandong Province ( BS2014NY009, ZR2013CM023 and ZR2015YL076 ), Funds for Distinguished Young Scientists of Shandong Province ( JQ201411 ), Special Fund for Agro-scientific Research in the Public Interest ( 201303046 and 2014CGPY04 ), Fund from Shandong Provincial Technology System of Modern Agricultural Industry ( SDAIT-06-022-06 ), and Taishan Scholars Project . DY is a recipient of the Agriculture and Food Research Initiative (AFRI) Competitive Grant no. 2013–67015-21243 of the US Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) . Publisher Copyright: {\textcopyright} 2016",

year = "2016",

month = dec,

day = "1",

doi = "10.1016/j.virol.2016.07.008",

language = "English (US)",

volume = "499",

pages = "278--287",

journal = "Virology",

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T1 - Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition

AU - Chen, Zhi

AU - Liu, Shaoning

AU - Sun, Wenbo

AU - Chen, Lei

AU - Yoo, Dongwan

AU - Li, Feng

AU - Ren, Sufang

AU - Guo, Lihui

AU - Cong, Xiaoyan

AU - Li, Jun

AU - Zhou, Shun

AU - Wu, Jiaqiang

AU - Du, Yijun

AU - Wang, Jinbao

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China ( 31170146 , 31602076 , 31370189 and 31572550 ), Research Award Fund for Outstanding Young Scientist of Shandong Province ( BS2014NY009, ZR2013CM023 and ZR2015YL076 ), Funds for Distinguished Young Scientists of Shandong Province ( JQ201411 ), Special Fund for Agro-scientific Research in the Public Interest ( 201303046 and 2014CGPY04 ), Fund from Shandong Provincial Technology System of Modern Agricultural Industry ( SDAIT-06-022-06 ), and Taishan Scholars Project . DY is a recipient of the Agriculture and Food Research Initiative (AFRI) Competitive Grant no. 2013–67015-21243 of the US Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) . Publisher Copyright: © 2016

PY - 2016/12/1

Y1 - 2016/12/1

N2 - The nonstructural protein 1α (NSP1α) of porcine reproductive and respiratory syndrome virus (PRRSV) is a nucleo-cytoplasmic protein that suppresses the production of type I interferon (IFN). In this study, we investigated the relationship between the subcellular distribution of NSP1α and its inhibition of type I IFN. NSP1α was found to contain the classical nuclear export signal (NES) and NSP1α nuclear export was CRM-1-mediated. NSP1α was shuttling between the nucleus and cytoplasm. We also showed that the nuclear export of NSP1α was necessary for its ability for type I IFN inhibition. NSP1α was also found to interact with CBP, which implies a possible mechanism of CBP degradation by NSP1α. Taken together, our results describe a novel mechanism of PRRSV NSP1α for type I IFN inhibition and suppression of the host innate antiviral response.

AB - The nonstructural protein 1α (NSP1α) of porcine reproductive and respiratory syndrome virus (PRRSV) is a nucleo-cytoplasmic protein that suppresses the production of type I interferon (IFN). In this study, we investigated the relationship between the subcellular distribution of NSP1α and its inhibition of type I IFN. NSP1α was found to contain the classical nuclear export signal (NES) and NSP1α nuclear export was CRM-1-mediated. NSP1α was shuttling between the nucleus and cytoplasm. We also showed that the nuclear export of NSP1α was necessary for its ability for type I IFN inhibition. NSP1α was also found to interact with CBP, which implies a possible mechanism of CBP degradation by NSP1α. Taken together, our results describe a novel mechanism of PRRSV NSP1α for type I IFN inhibition and suppression of the host innate antiviral response.

KW - NSP1α

KW - Nuclear export signal (NES)

KW - PRRSV

KW - Type I IFN

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UR - http://www.scopus.com/inward/citedby.url?scp=84989867145&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2016.07.008

DO - 10.1016/j.virol.2016.07.008

M3 - Article

C2 - 27718457

AN - SCOPUS:84989867145

SN - 0042-6822

VL - 499

SP - 278

EP - 287

JO - Virology

JF - Virology

ER -

Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition

Abstract

Keywords

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