Nuclear dynamics in metastatic cells studied by quantitative phase imaging

Silvia Ceballos, Mikhail Kandel, Shamira Sridharan, Freddy Monroy, Gabriel Popescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We used a new quantitative high spatiotemporal resolution phase imaging tool to explore the nuclear structure and dynamics of individual cells. We used a novel analysis tool to quantify the diffusion outside and inside the nucleus of live cells. We also obtained information about the nuclear spatio temporal mass density in metastatic cells. The results indicate that in the cytoplasm, the intracellular transport is mainly active (direct, deterministic), while inside the nucleus it is both active and passive (diffusive, random). We calculated the standard deviation of velocities in active transport and the diffusion coefficient for passive transport.

Original languageEnglish (US)
Title of host publicationQuantitative Phase Imaging
EditorsYongKeun Park, Gabriel Popescu
PublisherSPIE
ISBN (Electronic)9781628414264
DOIs
StatePublished - 2015
Event1st Conference on Quantitative Phase Imaging, QPI 2015 - San Francisco, United States
Duration: Feb 7 2015Feb 10 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9336
ISSN (Print)1605-7422

Other

Other1st Conference on Quantitative Phase Imaging, QPI 2015
Country/TerritoryUnited States
CitySan Francisco
Period2/7/152/10/15

Keywords

  • Quantitative phase imaging
  • biomedical optics
  • cell dynamics
  • diffusion
  • intracellular transport
  • microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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