Nuclear DNA analysis of maize seedlings treated with the triazole fungicide, triticonazole

Digambareppa P. Biradar, Wayne L. Pedersen, A Lane Rayburn

Research output: Contribution to journalArticle

Abstract

Maize kernels were treated with the fungicide triticonazole (RP‐727), belonging to the triazole family. The nuclear DNA content was analyzed in various maize plant organs by using flow cytometry. Two fluorochromes with different DNA binding mechanisms were used for probing nuclear DNA and chromatin structure. Results indicated nuclear alterations with the use of triticonazole and the alterations observed were different in the various tissues analyzed. Active meristematic plant organs, such as root tip, did not show any significant change in nuclear DNA, but chromatin structure was altered with increasing concentrations of fungicide. Stem and mesocotyl showed both DNA and chromatin condensation changes with triticonazole concentration, but the trends observed in these two plant organs were opposite. The magnitude of the alterations was minute in the different plant organs studied. These alterations do not appear to be deleterious for normal plant development.

Original languageEnglish (US)
Pages (from-to)291-295
Number of pages5
JournalPesticide Science
Volume41
Issue number4
DOIs
StatePublished - Aug 1994

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triazole fungicides
Triazoles
plant organs
Seedlings
nuclear genome
Zea mays
chromatin
corn
seedlings
DNA
Chromatin
fungicides
triazoles
fluorescent dyes
root tips
Plant Development
Meristem
flow cytometry
plant development
Fluorescent Dyes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Nuclear DNA analysis of maize seedlings treated with the triazole fungicide, triticonazole. / Biradar, Digambareppa P.; Pedersen, Wayne L.; Rayburn, A Lane.

In: Pesticide Science, Vol. 41, No. 4, 08.1994, p. 291-295.

Research output: Contribution to journalArticle

Biradar, Digambareppa P. ; Pedersen, Wayne L. ; Rayburn, A Lane. / Nuclear DNA analysis of maize seedlings treated with the triazole fungicide, triticonazole. In: Pesticide Science. 1994 ; Vol. 41, No. 4. pp. 291-295.
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