Nuclear and extranuclear pathway inputs in the regulation of global gene expression by estrogen receptors

Zeynep Madak-Erdogan, Karen J. Kieser, Hoon Kim Sung, Barry Komm, John A. Katzenellenbogen, Benita S Katzenellenbogen

Research output: Contribution to journalArticle

Abstract

Whereas estrogens exert their effects by binding to nuclear estrogen receptors (ERs) and directly altering target gene transcription, they can also initiate extranuclear signaling through activation of kinase cascades. We have investigated the impact of estrogen-mediated extranuclear-initiated pathways on global gene expression by using estrogen-dendrimer conjugates (EDCs), which because of their charge and size remain outside the nucleus and can only initiate extranuclear signaling. Genome-wide cDNA microarray analysis of MCF-7 breast cancer cells identified a subset of 17β-estradiol (E2)-regulated genes (∼25%) as EDC responsive. The EDC and E2-elicited increases in gene expression were due to increases in gene transcription, as observed in nuclear run-on assays and RNA polymerase II recruitment and phosphorylation. Treatment with antiestrogen or ERα knockdown using small interfering RNA abolished EDC-mediated gene stimulation, whereas GPR30 knockdown or treatment with a GPR30-selective ligand was without effect, indicating ER as the mediator of these gene regulations. Inhibitors of MAPK kinase and c-Src suppressed both E2 and EDC stimulated gene expression. Of note, in chromatin immunoprecipitation assays, EDC was unable to recruit ERα to estrogen-responsive regions of regulated genes, whereas ERα recruitment by E2 was very effective. These findings suggest that other transcription factors or kinases that are downstream effectors of EDC-initiated extranuclear signaling cascades are recruited to regulatory regions of EDC-responsive genes in order to elicit gene stimulation. This study thus highlights the importance of inputs from both nuclear and extranuclear ER signaling pathways in regulating patterns of gene expression in breast cancer cells.

Original languageEnglish (US)
Pages (from-to)2116-2127
Number of pages12
JournalMolecular Endocrinology
Volume22
Issue number9
DOIs
StatePublished - Sep 1 2008

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Gene Expression Regulation
Estrogen Receptors
Dendrimers
Estrogens
Genes
Gene Expression
Phosphotransferases
Breast Neoplasms
Gene Order
Estrogen Receptor Modulators
RNA Polymerase II
Chromatin Immunoprecipitation
Nucleic Acid Regulatory Sequences
Mitogen-Activated Protein Kinase Kinases
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Small Interfering RNA
Estradiol
Transcription Factors
Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Nuclear and extranuclear pathway inputs in the regulation of global gene expression by estrogen receptors. / Madak-Erdogan, Zeynep; Kieser, Karen J.; Sung, Hoon Kim; Komm, Barry; Katzenellenbogen, John A.; Katzenellenbogen, Benita S.

In: Molecular Endocrinology, Vol. 22, No. 9, 01.09.2008, p. 2116-2127.

Research output: Contribution to journalArticle

Madak-Erdogan, Zeynep ; Kieser, Karen J. ; Sung, Hoon Kim ; Komm, Barry ; Katzenellenbogen, John A. ; Katzenellenbogen, Benita S. / Nuclear and extranuclear pathway inputs in the regulation of global gene expression by estrogen receptors. In: Molecular Endocrinology. 2008 ; Vol. 22, No. 9. pp. 2116-2127.
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