Novel technique to isolate single specific drosophila larva CNS neuron

Ru Xin Li, Xin Zhu Yu, Xiang Ping He, Zuo Ping Xie

Research output: Contribution to journalArticlepeer-review

Abstract

Based on the traditional mechanical acute-isolation method, we developed a novel technique to isolate single specific drosophila larva CNS neuron by using two micro-manipulated glass pipettes with tungsten threads combined with fluorescence imaging. Under confocal and inversed fluorescence microscopies, we observed drosophila larva Murashka neurons, and successfully isolated one single Murashka neuron from drosophila larva. In the meantime, we recorded K current in this neuron using the patch-clamp technique. This novel technique is a powerful tool for isolation and investigation of fluorescence-labeled drosophila mutant CNS neurons.

Original languageEnglish (US)
Pages (from-to)152-155
Number of pages4
JournalHarbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology
Volume41
Issue number1
StatePublished - Jan 2009
Externally publishedYes

Keywords

  • CNS neuron
  • Drosophila
  • Mechanical acute isolation
  • Murashka
  • Patch-clamp

ASJC Scopus subject areas

  • General Engineering

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