Based on the traditional mechanical acute-isolation method, we developed a novel technique to isolate single specific drosophila larva CNS neuron by using two micro-manipulated glass pipettes with tungsten threads combined with fluorescence imaging. Under confocal and inversed fluorescence microscopies, we observed drosophila larva Murashka neurons, and successfully isolated one single Murashka neuron from drosophila larva. In the meantime, we recorded K current in this neuron using the patch-clamp technique. This novel technique is a powerful tool for isolation and investigation of fluorescence-labeled drosophila mutant CNS neurons.
|Original language||English (US)|
|Number of pages||4|
|Journal||Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology|
|State||Published - Jan 2009|
- CNS neuron
- Mechanical acute isolation
ASJC Scopus subject areas