Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts

Rasha Kanhoush, Brent Beenders, Caroline Perrin, Jacques Moreau, Michel Bellini, May Penrad-Mobayed

Research output: Contribution to journalArticle

Abstract

The heterogenous nuclear ribonucleoprotein G (hnRNP G) controls the alternative splicing of several pre-mRNAs. While hnRNP G displays an amino terminal RNA recognition motif (RRM), we find that this motif is paradoxically not implicated in the recruitment of hnRNP G to nascent transcripts in amphibian oocytes. In fact, a deletion analysis revealed that targeting of hnRNP G to active transcription units depends on another domain, centrally positioned, and consisting of residues 186-236. We show that this domain acts autonomously and thus is named NTD for nascent transcripts targeting domain. Furthermore, using an RNA probe previously characterized in vitro as an RNA that interacts specifically with hnRNP G, we demonstrate a new auxiliary RNA binding domain (RBD). It corresponds to a short region of 58 residues positioned at the carboxyl terminal end of the protein, which recognizes an RNA motif predicted to adopt an hairpin structure. The fact that the NTD acts independently from both the RRM and the RBD strongly suggests that the initial recruitment of hnRNP G to nascent pre-mRNAs is independent of its sequence-specific RNA binding properties. Together, these findings highlight the modular organization of hnRNP G and offer new insights into its multifunctional roles.

Original languageEnglish (US)
Pages (from-to)109-122
Number of pages14
JournalNucleus
Volume1
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Ribonucleoproteins
RNA
Proteins
RNA Precursors
RNA Probes
Nucleotide Motifs
Alternative Splicing
Amphibians
Oocytes

Keywords

  • Heterogeneous nuclear ribonucleoproteins
  • RNA binding domains
  • Transcription units
  • lampbrush chromosomes
  • oocyte

ASJC Scopus subject areas

  • Cell Biology

Cite this

Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts. / Kanhoush, Rasha; Beenders, Brent; Perrin, Caroline; Moreau, Jacques; Bellini, Michel; Penrad-Mobayed, May.

In: Nucleus, Vol. 1, No. 1, 01.01.2010, p. 109-122.

Research output: Contribution to journalArticle

Kanhoush, Rasha ; Beenders, Brent ; Perrin, Caroline ; Moreau, Jacques ; Bellini, Michel ; Penrad-Mobayed, May. / Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts. In: Nucleus. 2010 ; Vol. 1, No. 1. pp. 109-122.
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