@article{293bd2b9d46b462197f8f6d932e6f76c,
title = "Notch-dependent binary fate choice regulates the Netrin pathway to control axon guidance of Drosophila visual projection neurons",
abstract = "Notch-dependent binary fate choice between sister neurons is one of the mechanisms to generate neural diversity. How these upstream neural fate specification programs regulate downstream effector genes to control axon targeting and neuropil assembly remains less well understood. Here, we report that Notch-dependent binary fate choice in Drosophila medulla neurons is required to regulate the Netrin axon guidance pathway, which controls targeting of transmedullary (Tm) neurons to lobula. In medulla neurons of Notch-on hemilineage composed of mostly lobula-targeting neurons, Notch signaling is required to activate the expression of Netrin-B and repress the expression of its repulsive receptor Unc-5. Turning off Unc-5 is necessary for Tm neurons to target lobula. Furthermore, Netrin-B provided by Notch-on medulla neurons is required for correct targeting of Tm axons from later-generated medulla columns. Thus, the coordinate regulation of Netrin pathway components by Notch signaling ensures correct targeting of Tm axons and contributes to the neuropil assembly.",
keywords = "CP: Neuroscience, Drosophila medulla visual projection neurons, Netrin pathway, Notch-dependent binary fate choice, axon guidance",
author = "Yu Zhang and Scott Lowe and Ding, {Andrew Z.} and Xin Li",
note = "We thank the fly community, especially Greg J. Bashaw, Benjamin Altenhein, Yuh Nung Jan, C. Delidakis, Barry J. Dickson, and Claude Desplan for generous gifts of antibodies and fly stocks. We thank the Bloomington Drosophila Stock Center, the Vienna Drosophila RNAi Center, the Developmental Studies Hybridoma Bank, and TriP at Harvard Medical School (NIH/NIGMS R01-GM084947) for fly stocks and reagents. We thank Filipe Pinto-Teixeira, Isabel Holguera, and Neset Ozel for helpful discussions. This work was supported by the National Institutes of Health (grant nos. R01 EY026965-01A1 and R01 EY026965-06A0 to X.L.). Conceptualization, Y.Z. and X.L.; investigation, Y.Z. S.L. and A.Z.D.; formal analysis and visualization, Y.Z.; writing – original draft, Y.Z. and X.L.; writing – review & editing, Y.Z. X.L. S.L. and A.Z.D.; funding acquisition, X.L. The authors declare no competing interests. We thank the fly community, especially Greg J. Bashaw, Benjamin Altenhein, Yuh Nung Jan, C. Delidakis, Barry J. Dickson, and Claude Desplan for generous gifts of antibodies and fly stocks. We thank the Bloomington Drosophila Stock Center, the Vienna Drosophila RNAi Center, the Developmental Studies Hybridoma Bank, and TriP at Harvard Medical School (NIH/NIGMS R01-GM084947 ) for fly stocks and reagents. We thank Filipe Pinto-Teixeira, Isabel Holguera, and Neset Ozel for helpful discussions. This work was supported by the National Institutes of Health (grant nos. R01 EY026965-01A1 and R01 EY026965-06A0 to X.L.).",
year = "2023",
month = mar,
day = "28",
doi = "10.1016/j.celrep.2023.112143",
language = "English (US)",
volume = "42",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "3",
}