TY - JOUR
T1 - Nosema chrysorrhoeae n. sp. (Microsporidia), isolated from browntail moth (Euproctis chrysorrhoea L.) (Lepidoptera, Lymantriidae) in Bulgaria
T2 - Characterization and phylogenetic relationships
AU - Hyliš, Miroslav
AU - Pilarska, Daniela K.
AU - Oborník, Miroslav
AU - Vávra, Jiří
AU - Solter, Leellen F.
AU - Weiser, Jaroslav
AU - Linde, Andreas
AU - McManus, Michael L.
PY - 2006/2/1
Y1 - 2006/2/1
N2 - A new microsporidian parasite Nosema chrysorrhoeae n. sp., isolated in Bulgaria from the browntail moth (Euproctis chrysorrhoea L.), is described. Its life cycle includes two sequential developmental cycles that are similar to the general developmental cycles of the Nosema-like microsporidia and are indistinguishable from those of two Nosema spp. from Lymantria dispar. The primary cycle takes place in the midgut tissues and produces binucleate primary spores. The secondary developmental cycle takes place exclusively in the silk glands and produces binucleate environmental spores. N. chrysorrhoeae is specific to the browntail moth. Phylogenetic analysis based on the ssu rRNA gene sequence places N. chrysorrhoeae in the Nosema/Vairimorpha clade, with the microsporidia from lymantriid and hymenopteran hosts. Partial sequences of the lsu rRNA gene and ITS of related species Nosema kovacevici (Purrini K., Weiser J., 1975. Natürliche Feinde des Goldafters, Euproctis chrysorrhoea L., im Gebiet von Kosovo, FSR Jugoslawien. Anzeiger fuer Schädlingskunde, Pflanzen-Umweltschutz, 48, 11-12), Nosema serbica Weiser, 1963 and Nosema sp. from Lymantria monacha was obtained and compared with N. chrysorrhoeae. The molecular data indicate the necessity of future taxonomic reevaluation of the genera Nosema and Vairimorpha.
AB - A new microsporidian parasite Nosema chrysorrhoeae n. sp., isolated in Bulgaria from the browntail moth (Euproctis chrysorrhoea L.), is described. Its life cycle includes two sequential developmental cycles that are similar to the general developmental cycles of the Nosema-like microsporidia and are indistinguishable from those of two Nosema spp. from Lymantria dispar. The primary cycle takes place in the midgut tissues and produces binucleate primary spores. The secondary developmental cycle takes place exclusively in the silk glands and produces binucleate environmental spores. N. chrysorrhoeae is specific to the browntail moth. Phylogenetic analysis based on the ssu rRNA gene sequence places N. chrysorrhoeae in the Nosema/Vairimorpha clade, with the microsporidia from lymantriid and hymenopteran hosts. Partial sequences of the lsu rRNA gene and ITS of related species Nosema kovacevici (Purrini K., Weiser J., 1975. Natürliche Feinde des Goldafters, Euproctis chrysorrhoea L., im Gebiet von Kosovo, FSR Jugoslawien. Anzeiger fuer Schädlingskunde, Pflanzen-Umweltschutz, 48, 11-12), Nosema serbica Weiser, 1963 and Nosema sp. from Lymantria monacha was obtained and compared with N. chrysorrhoeae. The molecular data indicate the necessity of future taxonomic reevaluation of the genera Nosema and Vairimorpha.
KW - Euproctis chrysorrhoea
KW - Microsporidia
KW - Nosema chrysorrhoeae
KW - Nosema kovacevici
KW - Nosema serbica
KW - Taxonomy
KW - Ultrastructure
KW - rDNA phylogeny
UR - http://www.scopus.com/inward/record.url?scp=31944443245&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31944443245&partnerID=8YFLogxK
U2 - 10.1016/j.jip.2005.11.006
DO - 10.1016/j.jip.2005.11.006
M3 - Article
C2 - 16410011
AN - SCOPUS:31944443245
VL - 91
SP - 105
EP - 114
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
SN - 0022-2011
IS - 2
ER -